Sampling, Dilution, and Loading Device-coupled High-Performance Liquid Chromatography Method for Successive On-line Analyses of Major Carbohydrate Products in Immobilized Cellulase Hydrolysate of Paper Cellulose
-
- CHENG Cheanyeh
- Department of Chemistry, Chung Yuan Christian University
-
- CHANG Kuo-Chung
- Department of Chemistry, Chung Yuan Christian University
Search this article
Abstract
A device with direct sampling, dilution, and sample loading has been utilized to couple a vertically hanging immobilized cellulase bioreactor with a high-performance liquid chromatography system to make a successful on-line analysis of the 3 major carbohydrate products: glucose, xylose, and cellobiose in the cellulase hydrolysate. The coupled analysis system for successive on-line monitoring of the enzymatic hydrolysis of paper cellulose was stable over a period of 96 h. A comparative study involving 4 different quantification methods for the 3 carbohydrates was conducted. The internal standard calibration method with on-line dilution was observed to be the best procedure in terms of accuracy, precision, limit of detection, and systematic error reduction. With this method, the analysis for glucose had 99.9% accuracy, RSD below 2.4%, and a 2.1 ppm detection limit. Matrix matching was found important in the preparation of standard calibration curves with refractive index detection.
Journal
-
- Analytical Sciences
-
Analytical Sciences 23 (3), 305-310, 2007
The Japan Society for Analytical Chemistry
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390282679233188224
-
- NII Article ID
- 130004441424
- 10018823992
-
- NII Book ID
- AA10500785
-
- COI
- 1:CAS:528:DC%2BD2sXjtlektb0%3D
-
- ISSN
- 13482246
- 09106340
-
- NDL BIB ID
- 8671982
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed