High Magnification Microscopic System Focusing on Transparent Liquid/Liquid Interface Formed in Thin-Layer Two-Phase Microcell

• TSUKAHARA Satoshi

  Department of Chemistry, Graduate School of Science, Hiroshima University

• SUEHARA Michinori

  Department of Chemistry, Graduate School of Science, Hiroshima University

• FUJIWARA Terufumi

  Department of Chemistry, Graduate School of Science, Hiroshima University

Abstract

A thin-layer two-phase microcell was fabricated without an adhesive, which showed no fluorescence and resisted acids. The lower hollow of the microcell was thin (0.18 mm), which was in the range of the working distance of the used objective of high magnification (60×) and high numerical aperture (1.2). A reflection method using probe light was newly employed for focusing the objective on a transparent dodecane/water interface strictly with an inverted microscope. The system was applied to in situ fluorescence microscopic measurements of the dynamics of single DNA molecules (165600 base pairs) at the dodecane/water interface.

Journal

• Analytical Sciences

  Analytical Sciences 23 (3), 375-378, 2007

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390001204256443520
• NII Article ID: 10018824303
• NII Article ID: 130004441436
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.23.375
• COI: 1:CAS:528:DC%2BD2sXjtlekurs%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 8672136
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8672136
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.23.375.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.23.375/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/23/3/23_3_375/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed