Application of AXIMA-TOF2-Novel MALDI TOF/RTOF mass spectrometry for biopolymer analysis.

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  • Shima Keisuke
    Shimadzu Co., Ltd. Life Science Business unit, Analytical & Measuring instrument division
  • Ichimura Katsuhiko
    Shimadzu Co., Ltd. Life Science Business unit, Analytical & Measuring instrument division

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  • タンパク質分析のための最新質量分析装置　　ＡＸＩＭＡ‐ＴＯＦ｀２´―新規ＭＡＬＤＩ　ＴＯＦ／ＲＴＯＦ質量分析計による生体高分子分析
  • AXIMA-TOF2--新規MALDI TOF/RTOF質量分析計による生体高分子分析
  • AXIMA TOF2 シンキ MALDI TOF RTOF シツリョウ ブンセキケイ ニ ヨル セイタイ コウブンシ ブンセキ

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Abstract

With the development of proteomics, biological researchers are increasingly using mass spectrometer. Hence, it is required for the analytical instruments to analyze the many kinds of biomolecules immediately and easily. The AXIMA-TOF2 TM, a MALDI TOF-TOF system features a novel, dual ion gate capable of high resolution precursor ion selection and high energy CID cell. This instrument has a superior ability of protein identification. In addition, it can be applied to the study of different classes of biomolecules. The following three features show that AXIMA-TOF2 TM can contribute to the analysis of biomolecules.<br> 1) The unique curved field reflectron enables the acquisition of all PSD and CID fragment ion without stepping the reflectron voltage.<br> 2) High energy CID-MS/MS can generate and detect a large amount of valuable product ions to get structural information.<br> 3) The high performance monoPULSE ion gate can select target ion correctly from crowded MS spectra of complex samples such as mixtures and be able to measure MS/MS spectra.<br>

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