Lanthanide Complex Strategy for Detection and Separation of Histidine-tagged Proteins

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Author(s)

    • TSUKUBE Hiroshi
    • Department of Chemistry, Graduate School of Science, Osaka City University
    • YANO Keiko
    • Department of Chemistry, Graduate School of Science, Osaka City University
    • ISHIDA Akito
    • Department of Intelligence Science, Graduate School of Human and Environmental Science, Kyoto Prefectural University
    • SHINODA Satoshi
    • Department of Chemistry, Graduate School of Science, Osaka City University

Abstract

A series of trivalent lanthanide complexes were immobilized on SPR sensory chips and protein separation membranes. They worked as alternatives to transition-metal complexes in detection and separation of histidine-tagged ubiquitin protein. Since the highly coordinated complex between NTA ligand, Eu<SUP>3+</SUP> center, and hexahistidine moiety exhibited the enhanced luminescence, the present system provides a further possibility for use in in-situ optical protein imaging process.

Journal

  • Chemistry Letters

    Chemistry Letters 36(4), 554-555, 2007-04-05

    The Chemical Society of Japan

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Codes

  • NII Article ID (NAID)
    10018881250
  • NII NACSIS-CAT ID (NCID)
    AA00603318
  • Text Lang
    ENG
  • Article Type
    SHO
  • ISSN
    03667022
  • DOI
    10.1246/cl.2007.554
  • Data Source
    CJP  J-STAGE 
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