空気中真菌類の分子マーカーとしてのエルゴステロールの大量注入GC/MS分析 Gas Chromatography-mass Spectrometric Determination of Ergosterol as a Chemical Marker for Fungal Biomass of Indoor and Outdoor Air by Large-volume Sample Injection

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Large-volume sample injection technique was utilized for gas chromatography-mass spectrometric determination of ergosterol to estimate fungal biomass of indoor and outdoor air. Sample was collected with a low volume air sampler onto a filer paper for 24 hours and saponified in methanolic KOH. Ergosterol was extracted with <i>n</i>-hexane and then derivatized to its TMS ether. The ergosterol-TMS ether was stable for a month in a refrigerator.<br>The measurements of ergosterol in the samples were carried out by sample injection of 50 μ<i>l</i> using a large volume injector into GC/MS followed by SIM analysis using ions <i>m</i>/<i>z</i> 363, 337 and 468. The lowest detectable amounts of the TMS derivative of ergosterol was 5 pg (signal-to-noise ratio of 3, m/z 363).<br>Actual air samples had been collected monthly in Nara city from January to December throughout 2006. The samples of indoor and outdoor air contained ergosterol from 0.03 to 0.96 ng/m<sup>3</sup> and from 0.04 to 0.49 ng/m<sup>3</sup>, respectively.

収録刊行物

  • 環境化学 : journal of environmental chemistry

    環境化学 : journal of environmental chemistry 17(1), 47-52, 2007-03-25

    Japan Society for Environmental Chemistry

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各種コード

  • NII論文ID(NAID)
    10018894042
  • NII書誌ID(NCID)
    AN10547099
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    09172408
  • NDL 記事登録ID
    8756261
  • NDL 雑誌分類
    ZN5(科学技術--建設工学・建設業--都市工学・衛生工学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z16-2322
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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