Characterization of the High-Density Bacteria in Biological Phosphorus Removal Process by using Buoyant Density Separation

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  • KONDO TAKASHI
    Department of Chemical Engineering, Waseda University
  • EBIE YOSHITAKA
    Research Center for Material Cycles and Waste Management, National Institute for Environmental Studies
  • TSUNEDA SATOSHI
    Department of Chemical Engineering, Waseda University
  • INAMORI YUHEI
    Research Center for Material Cycles and Waste Management, National Institute for Environmental Studies

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  • 密度勾配遠心分離法による生物学的リン除去プロセスにおける高密度微生物の特定

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Abstract

To evaluate the effectiveness of the separation of polyphosphate-accumulating organisms (PAOs) and glycogen accumulating organism (GAOs)/G-bacteria from the phylogenetically high-diverse activated sludge using the buoyant density separation, activated sludge was collected from the acetate-fed SBR and the microbial community was determined. The activated sludge sample collected at the end of the aerobic phase was subjected to the buoyant density separation. The microbial communities of separated sludge were fingerprinted by terminal restriction fragment length polymorphism (T－RFLP) analysis. The microbial community analysis exhibited that Candidatus ‘Accumulibacter phosphatis’ known as PAOs, Defluvicoccus- relative G-bacteria and the group GB known as GAOs and some organisms playing an important role for nitrogen removal existed in the SBR. The T－RFLP analysis for each fraction of the buoyant density separation revealed that Cand. ‘Accumulibacter phosphatis’ was selectively concentrated in the high-density fraction. Although some unidentified peaks were also the prominent, Defluvicoccus vanus-relative G-bacteria were failed to concentrate. The unique T－RF derived from organisms including the group GB was the prominent in the high-density fraction. The Acidobacteria, the Bacteroidetes and the Nitrospira were disappeared in the high-density fraction. Even though some bacteria related to phosphorus removal were eliminated, most of bacterial strains which do not play an important role for phosphorus removal could be eliminated by the buoyant density separation.

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