Investigation of Fecal Microbial Community Structure Using 16s rDNA Sequence in Pigs Fed Fermented Liquid Feed

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  • 発酵リキッド飼料を給与した豚ふん便の16s rDNA配列を用いた菌叢解析
  • ハッコウ リキッド シリョウ オ キュウヨシタ ブタ フンベン ノ 16s rDNA ハイレツ オ モチイタ キンソウ カイセキ

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Abstract

A fecal microbial community structure in pigs fed fermented liquid feed was investigated. The phylogenetic diversity of the fecal microbial community in pigs was analyzed by the PCR-clone library study and PCR-DGGE analysis. The V3 region of 16s rDNA was amplified by PCR using total DNA extracted from pig feces as a template. A clone library comprising of 120 cloned 16s rDNA sequences was classified into 39 ribotypes by RFLP analysis, and all clones were found to belong to Bacteroides, Spirochaetes, and Firmicutes. The abundance ratio of Firmicutes increased greatly from 66.7% to 83.3% for a pig fed fermented liquid feed. From the results of PCR-DGGE, Pig26 clone that belongs to Ficmicutes showed a marked increase. Because the homology between 16s rDNA of a known bacterium and Pig26 clone was low (<86%) as analyzed by Blast, we could not identify the Pig26 clone. Therefore, it was impossible to presume a feeding effect of fermented liquid feed based on a dominant organism name. On the other hand, the gastric acid resistance of Lactobacillus plantarum A305 used as a starter was insufficient. Therefore, Lactobacillus plantarum A305 was hardly able to grow in the digestive organ. Taken all together, the change in a pig's microbial community due to Lactobacillus plantarum A305 was slight and that change did not influence the pig's health.

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