Electrophoretic Characterization Bovine Serum IgG<SUB>1</SUB> and IgG<SUB>2</SUB> and Clinical Application

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  • ABE Shogo
    Veterinary Clinical Training Center, Yamagata Prefecture Federation Agricultural Mutual Aid Association
  • KOMINE Ken-ichi
    (株) ティーセル研究所
  • WATANABE Daisaku
    Veterinary Clinical Training Center, Yamagata Prefecture Federation Agricultural Mutual Aid Association
  • ITAGAKI Masasi
    Veterinary Clinical Training Center, Yamagata Prefecture Federation Agricultural Mutual Aid Association
  • SAKAI Jyun-ichi
    Veterinary Clinical Training Center, Yamagata Prefecture Federation Agricultural Mutual Aid Association
  • TAMURA Keiji
    (株) 細菌化学研究所
  • KAMATA Shin-ichi
    日本獣医畜産大学獣医畜産学部

Bibliographic Information

Other Title
  • ウシ血清IgG<SUB>1</SUB>およびIgG<SUB>2</SUB>の電気泳動的性状と臨床応用
  • ウシ血清IgG1およびIgG2の電気泳動的性状と臨床応用
  • ウシ ケッセイ IgG1 オヨビ IgG2 ノ デンキ エイドウテキ セイジョ
  • Electrophoretic Characterization Bovine Serum IgG1 and IgG2 and Clinical Application

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Abstract

Bovine serum IgG1 and IgG2 were characterized by cellulose acetate electrophoresis (CAE), SDS polyacrylamide get electrophoresis (SDS-PAGE), thingel isoelectric focusing (T-IEF) and immuno electrophoresis. Both IgG1 and IgG2 showed a molecular weight of 150kd. By CAE and T-IEF, IgG1 and IgG2 were detected on β-globulin and r-globuline areas respectively, showing isoelectricpoints 5.2 to 5.7, and 6.6 to 8.2. While microkierdal, single radial immunodiffusion and turbidimetric immunoassay (TIA) for protein measurement gave almost the same values, TIA seemed to be recommendable for clinical application, because of simplicity, rapidity and reproducibility.

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