Simple and Rapid Determination of Hydrogen Peroxide Using Phosphine-based Fluorescent Reagents with Sodium Tungstate Dihydrate

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  • ONODA Maki
    Department of Analytical Chemistry, School of Pharmaceutical Sciences, Teikyo University
  • UCHIYAMA Takefumi
    Department of Analytical Chemistry, School of Pharmaceutical Sciences, Teikyo University
  • MAWATARI Ken-ichi
    Department of Analytical Chemistry, School of Pharmaceutical Sciences, Teikyo University
  • KANEKO Kiyoko
    Department of Analytical Chemistry, School of Pharmaceutical Sciences, Teikyo University
  • NAKAGOMI Kazuya
    Department of Analytical Chemistry, School of Pharmaceutical Sciences, Teikyo University

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Abstract

A simple batch method for the fluorometric determination of hydrogen peroxide using phosphine-based fluorescent reagents has been developed. A rapid, mild and selective derivatization reaction was achieved by adding sodium tungstate dihydrate to the reaction mixture of hydrogen peroxide and a phosphine-based fluorescent reagent. When 4-diphenylphosphino-7-methylthio-2,1,3-benzoxadiazole was used as a reagent, the derivatization reaction was completed after 2 min at room temperature. The calibration curve was linear between 12.5 and 500 ng hydrogen peroxide in a 10 µL sample solution. This method is accurate and has potential for on-line applications.

Journal

  • Analytical Sciences

    Analytical Sciences 22 (6), 815-817, 2006

    The Japan Society for Analytical Chemistry

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