Morphological Significance of Cladosporium Contaminants on Materials and Utensils in Contact with Food

• OHTA TOSHIKO

  National Institute of Health Sciences

• PARK BONG JOO

  National Institute of Health Sciences

• AIHARA MAKI

  National Institute of Health Sciences

• RI NORITOSHI

  Hygiene & Microbiology Research Center

• SAITO TOSHIKO

  Tokyo University of Agriculture

• SAWADA TAKUO

  Nippon Veterinary and Animal Science University

• TAKATORI KOSUKE

  National Institute of Health Sciences

Abstract

Cladosporium contaminants on materials and utensils that come into contact with food were morphologically investigated. The most common contaminants, C. cladosporioides and C. sphaerospermum, were detected on the samples. The morphological changes of the Cladosporium species were investigated by using stereoscopic, optical light, fluorescent, and scanning electron microscopes. Microscopically the Cladosporium contaminants were observed as aggregated dark brown spots, strongly pigmented, irregularly swollen, and in long chains. Using fluorescent microscopy, the Cladosporium mycelia were clearly stained with fluorescein diacetate as viable cells, but the old cells were mostly non-viable, as shown by staining with propidium iodide. The dynamics of the morphological changes showed that the penetrating mycelia were closely attached to the surface of the materials and utensils under investigation. These results provide information about the significance of Cladosporium contamination on materials and utensils in contact with food and may contribute to the control of fungal contamination.

Journal

• Biocontrol Science

  Biocontrol Science 11 (2), 55-60, 2006

  The Society for Antibacterial and Antifungal Agents, Japan

Keywords

• <I>Cladosporium</I>
• Contamination
• Morphological changes
• Food associated utensils

Details

• CRID: 1390001204464394240
• NII Article ID: 10019175001
• NII Book ID: AA11169621
• DOI: 10.4265/bio.11.55
• ISSN: 18840205
• ISSN: 13424815
• PubMed: 16789547
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed