Development of a PCR-based Method for the Detection of Enteric Myxozoans Causing the Emaciation Disease of Cultured Tiger Puffer

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  • Yanagida Tetsuya
    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Freeman Mark A.
    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Nomura Yoshinori
    Kumamoto Prefectural Fisheries Research Center
  • Takami Ikuo
    Nagasaki Prefectural Institute of Fisheries
  • Sugihara Yukitaka
    Nagasaki Prefectural Institute of Fisheries
  • Yokoyama Hiroshi
    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Ogawa Kazuo
    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo

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Other Title
  • PCRによるトラフグ腸管寄生粘液胞子虫の検出

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Abstract

A polymerase chain reaction (PCR) -based method was developed to detect enteric myxozoans which cause the emaciation disease of cultured tiger puffer Takifugu rubripes. Three primer sets were designed based on the sequences of small subunit ribosomal DNA of Enteromyxum leei, Enteromyxum fugu and Leptotheca fugu. All of the primer sets specifically amplified the target DNA of each species. The PCR successfully detected parasite DNA not only from the intestinal mucosa of killed fish but also from gut contents collected from live fish using a swab inserted into the anus. These PCR analyses were more sensitive to detection of parasites than microscopic observation on the intestinal imprints. The present PCR method is useful for rapid diagnosis of the myxosporean emaciation disease.

Journal

  • Fish Pathology

    Fish Pathology 40 (1), 23-28, 2005

    The Japanese Society of Fish Pathology

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