Cloning and Characterization of the Novel Chimeric Gene p53/FXR2 in the Acute Megakaryoblastic Leukemia Cell Line CMK11-5
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- Kanezaki Rika
- Department of Pediatrics, Hirosaki University School of Medicine
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- Toki Tsutomu
- Department of Pediatrics, Hirosaki University School of Medicine
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- Xu Gang
- Department of Pediatrics, Hirosaki University School of Medicine Department of Pediatrics, 2nd Affiliated Hospital of China Medical University
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- Narayanan Ramswamy
- Department of Biological Sciences, Florida Atlantic University
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- Ito Etsuro
- Department of Pediatrics, Hirosaki University School of Medicine
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Abstract
The loss of p53 function is a key event in tumorigenesis. Inactivation of p53 in primary tumors and cell lines is mediated by several molecular mechanisms, including deletions and rearrangements. However, generation of a p53 fusion gene has not yet been reported. Here we report a novel p53/an autosomal homolog of the fragile X mental retardation (FXR2) chimeric gene generated by an interstitial deletion. Western blot analyses have shown that the p53/FXR2 protein is indeed expressed in a Down syndrome-related acute megakaryoblastic leukemia cell line, CMK11-5 cells. To investigate the properties of the p53/FXR2 protein, we observed its subcellular localization. Flag-tagged expression vectors were transfected into COS-7 cells and the proteins were stained with an anti-Flag antibody. The p53/FXR2 protein was expressed at high levels in the cytoplasm, whereas wild-type p53 and FXR2 were localized primarily in the nucleus and in the periphery of the nucleus, respectively. Treatment with a topoisomerase II inhibitor, VP16, failed to induce expression of a p53 target gene, the cyclin-dependent kinase inhibitor p21WAF-1/CIP1, in CMK11-5 cells, and transient transfection analysis showed that the p53/FXR2 protein failed to transactivate the p21WAF-1/CIP1 promoter. These results suggest that the p53/FXR2 fusion protein lacks the ability of wild-type p53 to function as a transcription factor. The p53/FXR2 gene is the first reported p53 fusion gene.
Journal
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- The Tohoku Journal of Experimental Medicine
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The Tohoku Journal of Experimental Medicine 209 (3), 169-180, 2006
Tohoku University Medical Press
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Details 詳細情報について
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- CRID
- 1390001204239821824
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- NII Article ID
- 130004459499
- 10019253497
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- NII Book ID
- AA00863920
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- ISSN
- 13493329
- 00408727
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed