Characterization of vitamin A-storing cells in mouse fibrous kidneys using Cygb/STAP as a marker of activated stellate cells

• Kida Yujiro

  Department of Anatomy II, School of Dental Medicine, Tsurumi University Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University

• Asahina Kinji

  Department of Pathological Biochemistry, Medical Research Institute, Tokyo Medical and Dental University

• Inoue Kouji

  Research Center of Electron Microscopy, School of Dental Medicine, Tsurumi University

• Kawada Norifumi

  Department of Hepatology, Graduate School of Medicine, Osaka City University

• Yoshizato Katsutoshi

  Developmental Biology Laboratory, Department of Biological Science, Graduate School of Science, Hiroshima University

• Wake Kenjiro

  Department of Anatomy II, School of Dental Medicine, Tsurumi University Liver Research Unit, Minophagen Pharmaceutical Co. Ltd.

• Sato Tetsuji

  Department of Anatomy II, School of Dental Medicine, Tsurumi University Research Center of Electron Microscopy, School of Dental Medicine, Tsurumi University

抄録

The expression of the cytoglobin/stellate cell activation-associated protein (Cygb/STAP) was recently confirmed in all splanchnic vitamin A-storing cells - including hepatic stellate cells (HSCs) - in normal conditions. In the hepatic fibrous lesion, the expression of Cygb/STAP has been shown to be upregulated in activated HSCs and myofibroblasts (MFs), which have synthesized extracellular matrices. Furthermore, splanchnic vitamin A-storing cells have been reported to be distributed in the kidney. In this study, we clarify the contribution of vitamin A-storing cells to renal fibrosis by focusing on Cygb/ STAP. Adult mice were subjected to unilateral ureteral obstruction (UUO) and kidneys were harvested 1, 3, 7, and 10 days after UUO.<BR> Numbers of Cygb/STAP-immunopositive cells as well as Cygb/STAP mRNA 3 days after UUO (UUO day 3 kidney) increased. Vitamin A-autofluorescence was observed in intertubular spaces of controls but gradually declined in a time-dependent manner after UUO. Cygb/STAP⁺ cells were not completely identical with α-smooth muscle actin (αSMA)-positive cells in the control or UUO day 7 kidneys. Immunohistochemical analysis for Cygb/STAP and fibulin-2 (Fib), a specific marker for distinguishing MFs from activated HSCs, revealed that the number of Fib⁺STAP⁺ cells (MFs) and Fib-STAP⁺ cells (splanchnic vitamin A-storing cells) significantly increased in UUO day 3 and UUO day 7 kidneys compared with the controls. Our present findings support the concept that Cygb/STAP can be a unique marker for splanchnic fibroblast-like cells, namely the vitamin A-storing cell lineage, and suggest that splanchnic vitamin A-storing cells contribute to renal fibrogenesis in the obstructed kidney.

収録刊行物

• Ａｒｃｈｉｖｅｓ　ｏｆ　Ｈｉｓｔｏｌｏｇｙ　ａｎｄ　Ｃｙｔｏｌｏｇｙ

  Ａｒｃｈｉｖｅｓ　ｏｆ　Ｈｉｓｔｏｌｏｇｙ　ａｎｄ　Ｃｙｔｏｌｏｇｙ 70 (2), 95-106, 2007

  国際組織細胞学会

キーワード

• Histology

詳細情報

• CRID: 1390001206411875584
• NII論文ID: 10019550991
• NII書誌ID: AA1068990X
• DOI: 10.1679/aohc.70.95
• ISSN: 13491717
• ISSN: 09149465
• Web Site: http://www.jstage.jst.go.jp/article/aohc/70/2/70_2_95/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2008080750
• 本文言語コード: en
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