Altered Expression of Basement Membrane-related Molecules in Rat Brain Pericyte, Endothelial, and Astrocyte Cell Lines after Transforming Growth Factor-β1 Treatment

• KOSE Noriko

  Department of Pharmaceutics, Kyoritsu University of Pharmacy

• ASASHIMA Tomoko

  Department of Pharmaceutics, Kyoritsu University of Pharmacy CREST, Japan Science and Technology Corporation (JST) Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University New Industry Creation Hatchery Center, Tohoku University

• MUTA Mariko

  Department of Pharmaceutics, Kyoritsu University of Pharmacy

• IIZASA Hisashi

  Department of Pharmaceutics, Kyoritsu University of Pharmacy The Wistar Institute

• SAI Yoshimichi

  Department of Pharmaceutics, Kyoritsu University of Pharmacy

• TERASAKI Tetsuya

  CREST, Japan Science and Technology Corporation (JST) Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University New Industry Creation Hatchery Center, Tohoku University

• NAKASHIMA Emi

  Department of Pharmaceutics, Kyoritsu University of Pharmacy

Bibliographic Information

Other Title

• Altered expression of basement membrane-related molecules in rat brain pericyte, endothelial, and astrocyte cell lines after transforming growth factor-beta1 treatment

Abstract

  The basement membrane at the blood-brain barrier (BBB) plays important roles in maintaining the structure and function of capillary vessels. The BBB is constructed from endothelial cells, astrocytes and pericytes, but their interactions in the formation or maintenance of basement membrane have not been established. Transforming growth factor-β1 (TGF-β1) is known to increase fibronectin in brain capillary basement membrane with deposition of β-amyloid. We previously reported that the mRNA level of α-smooth muscle actin in a brain capillary pericyte cell line TR-PCT1 was increased by treatment with TGF-β1. In this study, expression of mRNAs encoding basement membrane-related molecules in TR-PCT1, a rat endothelial cell line TR-BBB13, and a type 2 astrocyte cell line TR-AST4 was evaluated by RT-PCR. The effects of TGF-β1 on expression of basement membrane-related genes in these cell lines were also examined. Fibronectin, MMP-9, tPA, TIMP-1, and PAI-l in TR-PCT1 were higher than in TR-BBB13 and TR-AST4. In TR-PCT1 treated with TGF-β1, collagen type IV, PAI-1, and MMP-9 were increased, and TIMP-2 was reduced. The change in PAI-1 mRNA was faster than those in MMP-9, TIMP-2, collagen type IV mRNAs. These results suggest that pericytes may be key cells in the maintenance of the basement membrane at the BBB.<br>

Journal

• Drug Metabolism and Pharmacokinetics

  Drug Metabolism and Pharmacokinetics 22 (4), 255-266, 2007

  The Japanese Society for the Study of Xenobiotics

Keywords

• brain pericyte
• brain endothelial cell
• astrocyte
• transforming growth factor-β1
• basement membrane

Details

• CRID: 1390001205181167232
• NII Article ID: 10019551700
• NII Book ID: AA1162652X
• DOI: 10.2133/dmpk.22.255
• COI: 1:CAS:528:DC%2BD2sXhtFKqsr3K
• ISSN: 18800920
• ISSN: 13474367
• Web Site: http://api.elsevier.com/content/article/PII:S1347436715302603?httpAccept=text/xml
• Web Site: http://api.elsevier.com/content/article/PII:S1347436715302603?httpAccept=text/plain
• Web Site: https://search.jamas.or.jp/link/ui/2008064708
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed