ラット脳周皮細胞, 内皮細胞, アストロサイトにおける Transforming growth factor-β1 (TGF-β1) に応答する基底膜関連物質の発現解析 Altered Expression of Basement Membrane-related Molecules in Rat Brain Pericyte, Endothelial, and Astrocyte Cell Lines after Transforming Growth Factor-β1 Treatment

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Abstract

  The basement membrane at the blood-brain barrier (BBB) plays important roles in maintaining the structure and function of capillary vessels. The BBB is constructed from endothelial cells, astrocytes and pericytes, but their interactions in the formation or maintenance of basement membrane have not been established. Transforming growth factor-β1 (TGF-β1) is known to increase fibronectin in brain capillary basement membrane with deposition of β-amyloid. We previously reported that the mRNA level of α-smooth muscle actin in a brain capillary pericyte cell line TR-PCT1 was increased by treatment with TGF-β1. In this study, expression of mRNAs encoding basement membrane-related molecules in TR-PCT1, a rat endothelial cell line TR-BBB13, and a type 2 astrocyte cell line TR-AST4 was evaluated by RT-PCR. The effects of TGF-β1 on expression of basement membrane-related genes in these cell lines were also examined. Fibronectin, MMP-9, tPA, TIMP-1, and PAI-l in TR-PCT1 were higher than in TR-BBB13 and TR-AST4. In TR-PCT1 treated with TGF-β1, collagen type IV, PAI-1, and MMP-9 were increased, and TIMP-2 was reduced. The change in PAI-1 mRNA was faster than those in MMP-9, TIMP-2, collagen type IV mRNAs. These results suggest that pericytes may be key cells in the maintenance of the basement membrane at the BBB.<br>

Journal

  • Drug Metabolism and Pharmacokinetics

    Drug Metabolism and Pharmacokinetics 22(4), 255-266, 2007-08-25

    The Japanese Society for the Study of Xenobiotics

References:  35

Cited by:  1

Codes

  • NII Article ID (NAID)
    10019551700
  • NII NACSIS-CAT ID (NCID)
    AA1162652X
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13474367
  • Data Source
    CJP  CJPref  J-STAGE 
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