Transformation of <i>Lyophyllum decastes</i> by particle bombardment

  • Sunagawa Masahide
    Department of Applied Microbiology, Forestry and Forest Products Research Institute
  • Murata Hitoshi
    Department of Applied Microbiology, Forestry and Forest Products Research Institute
  • Miyazaki Yasumasa
    Department of Applied Microbiology, Forestry and Forest Products Research Institute
  • Nakamura Masaya
    Department of Applied Microbiology, Forestry and Forest Products Research Institute

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Other Title
  • Transformation of Lyophyllum decastes by particle bombardment

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Abstract

<p>The basidiomycete Lyophyllum decastes was transformed by means of particle bombardment. We isolated five transformants under twelve conditions differing in the two parameters of target distance and helium pressure. The transformation frequency was one transformant/ μg DNA. In the transformants, plasmid DNAs were integrated into the genomic DNA and stably maintained. This is the first report on transformation of L. decastes by particle bombardment.</p>

Journal

  • Mycoscience

    Mycoscience 48 (3), 195-197, 2007

    The Mycological Society of Japan

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