Heterotrimeric Complex of Replication Protein A, a Single-Stranded DNA Binding Protein, from the Silkworm, <i>Bombyx mori</i>

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  • Sugahara Ryohei
    Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • Mon Hiroaki
    Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • Yamashita Jun
    Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • Mitsunobu Hitoshi
    Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • Man Lee Jae
    Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • Kawaguchi Yutaka
    Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • Koga Katsumi
    Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • Kusakabe Takahiro
    Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences

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  • Heterotrimeric complex of replication protein A, a single-stranded DNA binding protein, from the silkworm, Bombyx mori

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Abstract

Replication protein A (Rpa) is a heterotrimeric protein complex, composed of three tightly associated subunits of RPA70 (Rpa1), RPA32 (Rpa2) and RPA14 (Rpa3) in the case of human cells. The Rpa is known to be required for almost all aspects of cellular DNA metabolism. In the present study, we isolated and characterized the cDNAs orthologous to the Homo sapience RPA2 and RPA3, BmRPA2 and BmRPA3, respectively, from the silkworm, Bombyx mori. Each gene has a single conserved OB-fold domain. Although there was a higher level of homology among Rpa2s from different species, those of Rpa3s were very low. Furthermore, the analysis using yeast two-hybrid system revealed that the BmRpa2 and BmRpa3 form a tight heterotrimeric complex with BmRpa1. In addition, we confirmed the silkworm Rpa complex binds single-stranded DNA, but not double-stranded DNA.<br>

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