The Perivitelline Space-Forming Capacity of Mouse Oocytes is Associated with Meiotic Competence

DOI Web Site Web Site 被引用文献5件 参考文献61件
  • INOUE Azusa
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo
  • AKIYAMA Tomohiko
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo
  • NAGATA Masao
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo
  • AOKI Fugaku
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo

この論文をさがす

抄録

Although mouse oocytes progressively acquire meiotic competence during their growth in the ovaries, only half of full-grown oocytes can accomplish meiosis. Two types of full-grown oocytes have been reported on the basis of their chromatin configuration, the surrounded-nucleolus (SN) type and the non-surrounded-nucleolus (NSN) type. Therefore, full-grown oocytes collected from the ovaries of adult animals comprise a heterogeneous population; some oocytes are meiotically incompetent (NSN-type), and some are competent (SN-type). In the present study, we found that full-grown oocytes could be classified into two groups using the criterion of formation of the perivitelline space (PVS) after culture with 3-isobutyl-1-methylxanthine (IBMX) for 1 h. In oocytes with a PVS, actin-filled processes within zona pellucidae originating from cumulus cells were reduced, while they were rich in oocytes without a PVS, suggesting that a reduction in these processes contributes to PVS formation. PVS formation was highly correlated with meiotic competence and SN-type configuration. The results of this study demonstrate that PVS formation is a useful criterion for easily distinguishing between SN- and NSN-type oocytes, without injury to the cells.<br>

収録刊行物

被引用文献 (5)*注記

もっと見る

参考文献 (61)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ