シーベリー葉ポリフェノールによる高脂肪食投与雄マウスの抗肥満効果  [in Japanese] Anti-obesity Effects of Seaberry (Hippophae rhamnoides) Leaf Polyphenols (SBLPP) in Male Mice Fed a High-fat Diet  [in Japanese]

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Abstract

高脂肪食餌と同時にシーベリー葉ポリフェノール(SBLPP)飲料を8週間与えた雄マウスの体重は,コントロールよりも有意に低く,特に腹部の脂肪重量および肝臓重量は顕著に低かった.また,SBLPP飲料を与えた雄マウスの血中GPT活性はコントロールよりも有意に低く,高脂肪食摂取による肝臓への脂肪蓄積の抑制が示された.また,8週間SBLPP飲料を与えた雄マウスの脂肪酸β酸化酵素であるacyl-CoA oxidase(ACO)およびmedium-chain acyl-CoA dehydrogenase(MCAD)の遺伝子発現量は有意に増加していた.<I>in vitro</I>実験により,SBLPPが膵リパーゼ活性を濃度依存的に抑制することが示され,そのIC<SUB>50</SUB>は4.5ppmを示した.これらの結果より,長期間,高脂肪食餌と同時にSBLPP飲料を雄マウスに与えると,肝臓における脂肪酸β酸化酵素の遺伝子発現が促進されて脂質代謝促進が起こると共に,膵リパーゼ活性を抑制することにより,体内への脂肪輸送が低下して,糞中に脂肪が排泄され,体脂肪蓄積が抑制されたと推定した.

The mean body weight of male mice fed a high-fat diet and seaberry leaf polyphenols (SBLPP) juice for eight weeks was significantly lower than that of controls, with the weights of abdominal adipose tissue and liver being particularly low. Additionally, blood GTP activity in experimental mice was significantly lower than in controls, demonstrating the inhibitory effects of SBLPP juice on development of fatty liver, which may otherwise be induced by a high-fat diet. Furthermore, expression of acyl-CoA oxidase (ACO), medium-chain acyl-CoA dehydrogenase (MCAD), and fatty acid beta-oxidization enzymes significantly increased in experimental mice. <I>In vitro</I> experiments showed that SBLPP juice suppressed pancreatic lipase activity in a concentration-dependent manner, with IC<SUB>50</SUB> being 4.5ppm. It was therefore suggested that, in mice fed a high-fat and SBLPP juice diet, gene expression of fatty acid beta-oxidation enzymes in the liver was enhanced, which increased the lipid metabolism and inhibited the pancreatic lipase activity, thus reducing lipid transportation to the body and increasing fecal lipid excretion. As a result, body fat deposition was inhibited.

Journal

  • Nippon Shokuhin Kagaku Kogaku Kaishi

    Nippon Shokuhin Kagaku Kogaku Kaishi 54(11), 477-481, 2007-11-15

    Japanese Society for Food Science and Technology

References:  25

Cited by:  1

Codes

  • NII Article ID (NAID)
    10019803515
  • NII NACSIS-CAT ID (NCID)
    AN10467499
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    1341027X
  • NDL Article ID
    9253413
  • NDL Source Classification
    ZP34(科学技術--食品工学・工業)
  • NDL Call No.
    Z17-126
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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