Liposome-Assisted Refolding of Microbial Transglutaminase

• Umakosh Hiroshi

  Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University

• Yoshimoto Noriko

  Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University

• Yoshimoto Makoto

  Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University

• Shimanouchi Toshinori

  Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University

• Kuboi Ryoichi

  Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University

Abstract

The liposome-assisted refolding of recombinant microbial transglutaminase (MTG) was achieved by using the dilution method. The analysis of the partly-denatured MTG by using the calcein-trapping immobilized liposome chromatography showed the interaction between the MTG and liposome under the above condition. The effect of the addition of liposome was then investigated, resulting that the refolding yield of MTG was improved from 66% at control to 82% by adding the liposome with suitable membrane fluidity and surface charge density.

Journal

• MEMBRANE

  MEMBRANE 32 (5), 287-293, 2007

  THE MEMBRANE SOCIETY OF JAPAN

Keywords

• liposome
• protein refolding
• immobilized liposome chromatography
• artificial chaperone

Details

• CRID: 1390282681398091264
• NII Article ID: 130007331135
• NII Article ID: 10019834184
• NII Book ID: AN0023215X
• DOI: 10.5360/membrane.32.287
• ISSN: 18846440
• ISSN: 03851036
• ISSN: http://id.crossref.org/issn/03851036
• NDL BIB ID: 8961309
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8961309
• Web Site: https://www.jstage.jst.go.jp/article/membrane/32/5/32_287/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2008069214
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed