Immobilized-Liposome Sensor System for Detection of Proteins under Stress Conditions

Access this Article

Author(s)

Abstract

An electrolyte-entrapping liposome was immobilized on an Au electrode to design and develop an immobilized-liposome sensor (ILS) system for the detection of the damage of proteins. Three kinds of proteins, such as bovine carbonic anhydrase, lysozyme and chitosanase, were used as model analytes. The proteins treated by a denaturant(guanidium hydrochloride, GuHCl) were applied to the ILS analysis, resulting that the significant electrical signalscould effectively be detected due to the interaction between the liposome and GuHCl-denatured protein. The diffusion currents normalized by protein concentrations were linearly correlated with local hydrophobicity of proteinsevaluated by the conventional aqueous two-phase partitioning method. The ILS system can, therefore, be utilized asa useful tool for an on-line monitoring to conformational changes of proteins.

Journal

  • MEMBRANE

    MEMBRANE 32(5), 294-301, 2007-09-01

    THE MEMBRANE SOCIETY OF JAPAN

References:  19

Cited by:  8

Codes

  • NII Article ID (NAID)
    10019834207
  • NII NACSIS-CAT ID (NCID)
    AN0023215X
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    03851036
  • NDL Article ID
    8961347
  • NDL Source Classification
    ZR2(科学技術--生物学--生化学)
  • NDL Call No.
    Z18-1127
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
Page Top