Relationship between the picolinic acid-induced potentiation of macrophage antimycobacterial activity and macrophage apoptosis

DOI 14 References
  • Tatano Yutaka
    Department of Microbiology and Immunology, Shimane University School of Medicine
  • Shimizu Toshiaki
    Department of Microbiology and Immunology, Shimane University School of Medicine
  • Yasumoto Ko
    Department of Microbiology and Immunology, Shimane University School of Medicine
  • Tomioka Haruaki
    Department of Microbiology and Immunology, Shimane University School of Medicine

Bibliographic Information

Other Title
  • ピコリン酸によるマクロファージのＭｙｃｏｂａｃｔｅｒｉｕｍ　ａｖｉｕｍ殺菌能増強作用とアポトーシスとの関連性

Search this article

Abstract

It has been reported that picolinic acid (PA) potentiated the antimicrobial activity of mouse bone marrowderived macrophages (Mφs) against intracellular Mycobacterium avium complex (MAC) and that such PA effects were accompanied by PA-induced Mφ apoptosis. It is of interest to examine whether or not such a phenomenon is also observed for human Mφs. In the present study, we examined PA activity in inducing apoptosis of THP-1 human Mφs (THP-1 Mφs) using the Annexin V and DNA laddering assay methods. Flow cytometric studies on test Mφs stained with Annexin V demonstrated that PA treatment induced phosphatidylserine translocation to the outer leaflet of the cell membrane THP-1 Mφs with or without M. avium infection. On the other hand, PA treatment did not cause significant levels of DNA laddering, even when test THP-1 Mφs were infected with M. avium. These findings suggest that PA may induce the early phase apoptotic events in THP-1 Mφs, without inducing DNA fragmentation characteristic to the middle to late phase apoptosis. Our present results provide insights into the possible role of Mφ apoptosis in the PA-induced potentiation of Mφ anti-MAC anitmicrobial activity.

Journal

References(14)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top