Characterization of Norovirus-Binding Proteins (NoVBPs) Recovered from Activated Sludge Microorganisms

• WADA Keishi

  Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University

• SANO Daisuke

  Department of Microbiology, School of Biology, University of Barcelona

• IMAI Takahiro

  Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University

• OMURA Tatsuo

  Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University

Bibliographic Information

Other Title

• 活性汚泥細菌から分離されたノロウイルス吸着タンパク質（Ｎｏｒｏｖｉｒｕｓ‐Ｂｉｎｄｉｎｇ　Ｐｒｏｔｅｉｎｓ：　ＮｏＶＢＰｓ）の特性評価
• 活性汚泥細菌から分離されたノロウイルス吸着タンパク質 (Norovirus-Binding Proteins: NoVBPs) の特性評価
• カッセイ オデイ サイキン カラ ブンリサレタ ノロウイルス キュウチャク タンパクシツ Norovirus Binding Proteins NoVBPs ノ トクセイ ヒョウカ

Abstract

In this study, norovirus-binding proteins (NoVBPs) were newly recovered from activated sludge culture using affinity chromatography. The C-terminal amino acid sequence (12 residues) of the Lordsdale virus, belonging to the dominant genotype (GII.4) of noroviruses throughout the world, was used as a ligand in the affinity chromatography. Two-dimensional electrophoresis analysis shows that the samples recovered by the affinity chromatography include a variety of proteins, which have a molecular weights between 60 and 10 kDa and isoelectric points between 3 and 6. The binding affinity of NoVBPs to the virus-like particle (VLP) of norovirus GII.4 was higher than that to the VLP of norovirus GI.1, which was indicated by ELISA. A determination of the N-terminal amino acid sequences revealed that several NoVBPs are relatives to a chaperonine protein, GroEL, which can interact with denatured hydrophobic proteins. The NoVBPs recovered in this study might be useful as versatile adsorbents for norovirus recovery or detection from environmental water samples.

Journal

• Journal of Japan Society on Water Environment

  Journal of Japan Society on Water Environment 30 (12), 731-736, 2007

  Japan Society on Water Environment

Keywords

• Norovirus
• norovirus-binding proteins (NoVBPs)
• affinity chromatography
• virus-like particles (VLPs)
• N-terminal amino acid sequence

Details

• CRID: 1390001205079953920
• NII Article ID: 10020008499
• NII Book ID: AN10372439
• DOI: 10.2965/jswe.30.731
• COI: 1:CAS:528:DC%2BD1cXhsVSitA%3D%3D
• ISSN: 18813690
• ISSN: 09168958
• HANDLE: 10097/52885
• NDL BIB ID: 9309910
• Web Site: https://tohoku.repo.nii.ac.jp/records/26795
• Web Site: http://id.ndl.go.jp/bib/9309910
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9309910
• Web Site: https://www.jstage.jst.go.jp/article/jswe/30/12/30_12_731/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed