Profiles of Intracellular Expression, Distribution, and Activation of Phospholipase A<SUB>2</SUB> in Host Macrophages Infected with <I>Mycobacterium avium</I> complex

• SANO Chiaki

  Department of Microbiology and Immunology Department of Oto -Rhino-Laryngology, Shimane University School of Medicine

• SHIMIZU Toshiaki

  Department of Microbiology and Immunology

• TATANO Yutaka

  Department of Microbiology and Immunology

• YASUMOTO Ko

  Department of Microbiology and Immunology

• TOMIOKA Haruaki

  Department of Microbiology and Immunology

Bibliographic Information

Other Title

• <I>Mycobacterium avium</I> complex感染マクロファージにおけるフォスフォリパーゼA<SUB>2</SUB>の細胞内動態
• Mycobacterium avium complex感染マクロファージにおけるフォスフォリパーゼA2の細胞内動態
• Mycobacterium avium complex カンセン マクロファージ ニ オケル フォスフォリパーゼ A2 ノ サイボウナイ ドウタイ
• Profiles of Intracellular Expression, Distribution, and Activation of Phospholipase A2 in Host Macrophages Infected with Mycobacterium avium complex

Abstract

Our recent studies have shown that type IV cytosolic phospholipase A₂ (cPLA₂) plays an important role in the expression of macrophage (MΦ) antimicrobial activity against the Mycobacterium avium complex (MAC). To clarify the modes of c PLA₂ participation in MΦ anti-MAC antimicrobial function in detail, we studied intracellular profiles of phospholilase A₂, focusing on c PLA₂, in MAC-infected MΦs, with the following findings: In murine peritoneal MΦs, cPLA₂ was constitutively expressed even in uninfected MΦs, and MAC infection did not increase intramacrophage c PLA₂ expression. In an RAW264.7 mouse MΦ cell line (RAW264.7 MΦs) infected with MAC, a portion of intracellular c PLA₂ was concentrated in MAC organisms residing within MΦ phagosomes. MAC infection upregulated intramacrophage c PLA₂ expression and induced its phosphorylation. These findings suggest that MAC infection of RAW264.7 MΦs may induce the activation of intracellular c PLA₂, translocating it to phagosomes engulfing infected MAC organisms.

Journal

• Kansenshogaku Zasshi

  Kansenshogaku Zasshi 81 (6), 695-699, 2007

  The Japanese Association for Infectious Diseases

Keywords

• <I>Mycobacterium avium</I> complex
• macrophage
• phospholipase A<SUB>2</SUB>

Details

• CRID: 1390282680026221440
• NII Article ID: 10020105809
• NII Article ID: 130004331409
• NII Book ID: AN00047715
• DOI: 10.11150/kansenshogakuzasshi1970.81.695
• COI: 1:STN:280:DC%2BD2sjlslOitQ%3D%3D
• ISSN: 1884569X
• ISSN: 03875911
• ISSN: http://id.crossref.org/issn/03875911
• NDL BIB ID: 9301744
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9301744
• Web Site: https://search.jamas.or.jp/link/ui/2008117753
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed