Gene expression profiling of synovial fibroblasts from human temporomandibular joint on stimulation with tumor necrosis factor-.ALPHA.
-
- AKUTSU Miwa
- Department of Maxillofacial Surgery, Nihon University School of Dentistry
-
- OGURA Naomi
- Department of Maxillofacial Surgery, Nihon University School of Dentistry Research Institute of Oral Science, Nihon University School of Dentistry
-
- SAKAMAKI Hiroyuki
- Department of Maxillofacial Surgery, Nihon University School of Dentistry Research Institute of Oral Science, Nihon University School of Dentistry
-
- TOBE Makiko
- Department of Maxillofacial Surgery, Nihon University School of Dentistry Research Institute of Oral Science, Nihon University School of Dentistry
-
- ITO Ko
- Department of Maxillofacial Surgery, Nihon University School of Dentistry Research Institute of Oral Science, Nihon University School of Dentistry
-
- ABIKO Yoshimitsu
- Research Institute of Oral Science, Nihon University School of Dentistry Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry
-
- KONDOH Toshirou
- Department of Maxillofacial Surgery, Nihon University School of Dentistry Research Institute of Oral Science, Nihon University School of Dentistry
Bibliographic Information
- Other Title
-
- 顎関節内障患者滑膜細胞のTNF‐α刺激による遺伝子発現プロファイリング
- ガク カンセツ ナイショウ カンジャ カツマク サイボウ ノ TNF a シゲキ ニ ヨル イデンシ ハツゲン プロファイリング
Search this article
Abstract
Tumor necrosis factor-α(TNF-α) plays a key role in pathological conditions of the temporomandibular joint (TMJ). However, the molecular mechanisms by which TNF-α contributes to synovial inflammation remain unclear. In this study, the gene expression profile of synovial fibroblasts stimulated by TNF-α was studied by Gene Chip analysis. Synovial fibroblasts were prepared from the tissues of three patients with internal derangement of the TMJ, using the outgrowth method. The synovial cells were stimulated by TNF-α for 4 hours, after which total RNA was extracted with TRlzol. Gene expression profiling was performed using the Affymetrix Gene Chip (Human Genome Focus Array, 8500 genes). Hybridization data were analyzed with Gene Spring software.<BR>Two hundred forty-five genes were found to be TNF-α responsive, showing a greater than two-fold difference in average intensity between the stimulated and control samples. The gene ontology classification was the “transregulator”, “catalytic activity”, “transcription regulator”, and “chaperone activity” categories. Among the top 25 up-regulated genes, 8 were found to be chemokines. The expression of chemokines in TNF-α responsive genes measured by Gene Chip was confirmed by real time-PCR. Chemokine gene expression was found to be increased by TNF-α.<BR>These results suggest that TNF-α may contribute to inflammation in internal derangement of the TMJ by producing chemokines.
Journal
-
- Japanese Journal of Oral and Maxillofacial Surgery
-
Japanese Journal of Oral and Maxillofacial Surgery 53 (9), 538-544, 2007
Japanese Society of Oral and Maxillofacial Surgeons
- Tweet
Details 詳細情報について
-
- CRID
- 1390282681509944448
-
- NII Article ID
- 10020114575
- 10031034051
-
- NII Book ID
- AN00189163
-
- ISSN
- 21861579
- 00215163
-
- NDL BIB ID
- 8954679
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
-
- Abstract License Flag
- Disallowed