"Drop-and-Sip" Fluid Handling Technique for the Reagent-Release Capillary (RRC)-based Capillary-Assembled Microchip (CAs-CHIP): Sample Delivery Optimization and Reagent Release Behavior in RRC
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- HENARES Terence G.
- Graduate School of Material Science, University of Hyogo
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- MIZUTANI Fumio
- Graduate School of Material Science, University of Hyogo
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- SEKIZAWA Ryuichi
- Metaboscreen Co. Ltd.
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- HISAMOTO Hideaki
- Graduate School of Engineering, Department of Applied Chemistry, Osaka Prefecture University
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Abstract
The experimental conditions of the sample delivery inside the reagent-release capillary-based capillary-assembled microchip (RRC-based CAs-CHIP) were optimized and the reagent release procedure in the RRC is discussed. Recently, our group introduced the basic concept of the “drop-and-sip” fluid handling technique (Anal. Chem., 2007, 79, 908). A microliter volume of sample solution is dropped on the inlet hole and is sipped into another hole, producing a sample plug flow in the main poly(dimethyl siloxane) (PDMS) channel, concurrently filling each sensing capillary that faces the main PDMS channel. However, the detailed evaluation of the successful sample delivery condition and the reagent release behavior in the RRC has not been fully discussed. Under our experimental conditions, ca. 0.6 - 2.4 s of sample plug-RRC contact time allowed the successful sample introduction into the RRC by capillary force without any reagent leakage or disturbance of the sample plug flow. On the other hand, reagent release behavior inside the RRC is governed by both convective and diffusive mass transport, which leads to a faster mixing time of the sample with reagents immobilized inside the RRC compared to that expected from the simple diffusion alone.
Journal
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- Analytical Sciences
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Analytical Sciences 24 (1), 127-132, 2008
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204255309568
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- NII Article ID
- 130004441554
- 10020143686
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD1cXpsFKntQ%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 9326872
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- PubMed
- 18187861
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed