Efficient Strontium-Induced Activation of Mouse Oocytes in Standard Culture Media by Chelating Calcium

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Without using sperm, artificial oocyte activation is essential for current assisted reproductive technologies, particularly somatic cell nuclear transfer and round spermatid injection. Strontium has been widely used as an activator of oocytes especially in the mouse, by which efficient oocyte activation requires Ca<sup>2+</sup>-free medium. In this study, we examined whether Sr<sup>2+</sup> can efficiently activate oocytes in Ca<sup>2+</sup>-containing culture media when calcium is chelated. Ethylene glycol-bis (β-aminoethyl ether) -N, N, N', N'-tetraacetic acid (EGTA) was added to three standard culture media (CZB, M16 and KSOM) for mouse embryos because it preferentially binds Ca<sup>2+</sup> rather than Sr<sup>2+</sup>. We found that treatment with 5 mM Sr<sup>2+</sup> and 2 mM EGTA left fewer than 1% of oocytes at the MII stage, which is comparable to that of Ca<sup>2+</sup>-free medium. As a result, addition of 2 mM EGTA along with 5 mM Sr<sup>2+</sup> in either CZB, M16 or KSOM made more than 80% of available activated oocytes, which was comparable to or better than 72% in a Ca<sup>2+</sup>-free Sr<sup>2+</sup> medium, since EGTA-Sr<sup>2+</sup> activation led to significantly less oocyte degeneration than Ca<sup>2+</sup>-free Sr<sup>2+</sup> activation. Furthermore, we demonstrated that this activation method can support the birth of cloned embryos. Thus, addition of EGTA to typical Ca<sup>2+</sup>-containing culture media can easily produce activation media that does not interfere with embryonic development.<br>

収録刊行物

  • The Journal of reproduction and development

    The Journal of reproduction and development 53(6), 1207-1215, 2007-12-01

    日本繁殖生物学会

参考文献:  34件中 1-34件 を表示

被引用文献:  4件中 1-4件 を表示

各種コード

  • NII論文ID(NAID)
    10020172730
  • NII書誌ID(NCID)
    AA10936678
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168818
  • NDL 記事登録ID
    9327021
  • NDL 雑誌分類
    ZR22(科学技術--農林水産--畜産)
  • NDL 請求記号
    Z54-H305
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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