青汁製品中のビタミンKの分析 Determination of Vitamin K in Aojiru (Green Juice) Products by HPLC

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市販青汁41製品について,ビタミンKの分析を行い,製品中の含有量と一日摂取量を調査した.青汁製品中のビタミンKは<i>n</i>-ヘキサンを用いて直接抽出し,HPLC試験溶液とした.ビタミンKはODSカラムで分離した後,白金黒カラムで還元して得られる蛍光(励起波長240 nm,蛍光波長430 nm)により測定した.その結果,製品中のビタミンK<sub>1</sub>含有量は冷凍製品90∼190 μg/100 g,粉末製品410∼3,300 μg/100 g,粒状製品640∼3,100 μg/100 gであり,各種青汁製品を喫食した場合,一日あたり20∼380 μgのビタミンK<sub>1</sub>を摂取すると推定された.ワーファリン服用者の場合,青汁製品の喫食がワーファリンの投薬効果に影響を与える可能性があることが明らかとなった.

A survey of vitamin K contents was carried out on 41 aojiru products and 10 vegetable juice products that were purchased from local markets. Aojiru is a health food made from green vegetables such as kale, ashitaba, mulberry leaf, barley grass and the like. The products are usually provided in various forms, such as frozen, powder and tablet.<br>Vitamin K in samples was extracted with <i>n</i>-hexane, and separated on a C18 column with methanol-ethanol (95 : 5). After separation, vitamin K was converted to the hydroquinone form on a reduction column and determined with a fluorescence detector at λ<sub>ex</sub> 240 nm and λ<sub>em</sub> 430 nm.<br>The contents of vitamin K<sub>1</sub> (phylloquinone) in frozen samples (<i>n</i>=8), powder samples (<i>n</i>=26) and tablet samples (<i>n</i>=7) were 90-190, 410-3,300, and 640-3,100 μg/100 g, respectively, and that in vegetable juice (<i>n</i>=10) was 1-12 μg/100 g. Vitamin K<sub>2</sub> (menaquinone) was not detected. The daily intake of vitamin K from aojiru products was estimated to be 99-380, 20-250 and 27-210 μg/day for frozen, powder and tablet types, respectively. These results suggest that patients prescribed warfarin should take care about their intake of vitamin K from aojiru products.

収録刊行物

  • 食品衛生学雑誌 = Journal of the Food Hygienics Society of Japan

    食品衛生学雑誌 = Journal of the Food Hygienics Society of Japan 47(2), 85-88, 2006-04-25

    [日本食品衛生学会]

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各種コード

  • NII論文ID(NAID)
    10020350364
  • NII書誌ID(NCID)
    AN00117741
  • 本文言語コード
    JPN
  • 資料種別
    NOT
  • ISSN
    00156426
  • NDL 記事登録ID
    7902625
  • NDL 雑誌分類
    ZS17(科学技術--医学--衛生学・公衆衛生)
  • NDL 請求記号
    Z19-364
  • データ提供元
    CJP書誌  CJP引用  NDL  IR  J-STAGE 
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