Possible Roles of Intracellular Cyclic AMP, Protein Kinase C and Calcium Ion in the Apoptotic Signaling Pathway in Bovine Luteal Cells

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Author(s)

    • KOMIYAMA Junichi
    • Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
    • ACOSTA Tomas J.
    • Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
    • OKUDA Kiyoshi
    • Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University

Abstract

Structural luteolysis occurs by apoptosis of luteal cells. The present study examined the effects of activators of well-characterized second messengers on <i>Fas</i> and <i>caspase-3</i> mRNA expression and on P4 production in luteal cells in order to trace the pro- and anti-apoptotic factors in the bovine corpus luteum (CL). Cultured bovine mid luteal cells were treated for 24 h with a cyclic AMP analogue (8-bromo cyclic AMP; 8br-cAMP; 2.5 mM), a protein kinase C (PKC) activator (phorbol 12-myristate 13-acetate; PMA; 10 <i>μ</i>M), or calcium ionophore (A23187; 10 μM). <i>Fas</i> and <i>caspase-3</i> mRNA expression was inhibited by 8br-cAMP and PMA but was increased by A23187 (P<0.05). In addition, P4 production by bovine luteal cells was stimulated by 8br-cAMP and PMA, whereas it was inhibited by A23187, compared with untreated controls (P<0.05). The overall results suggest that cAMP and PKC suppress apoptosis in bovine luteal cells through inhibition of <i>Fas</i> and <i>caspase-3</i> mRNA expression and through stimulation of P4 production. Therefore, substances that activate cAMP or PKC may act as survival factors in the bovine CL. Furthermore, substances that mobilize Ca<sup>2+</sup> may act as apoptotic factors by stimulating <i>Fas</i> and <i>caspase-3</i> expression in the bovine luteal cells.<br>

Journal

  • Journal of Reproduction and Development

    Journal of Reproduction and Development 52(4), 517-522, 2006-08-01

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

References:  31

Cited by:  2

Codes

  • NII Article ID (NAID)
    10020351661
  • NII NACSIS-CAT ID (NCID)
    AA10936678
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09168818
  • NDL Article ID
    8019826
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z54-H305
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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