Simple Prediction of the Survival of Follicles in Cryopreserved Human Ovarian Tissue
-
- MALTARIS Theodoros
- University Hospital Erlangen, Department of Obstetrics and Gynaecology
-
- DRAGONAS Charalampos
- University Hospital Erlangen, Department of Obstetrics and Gynaecology
-
- HOFFMANN Inge
- University Hospital Erlangen, Department of Obstetrics and Gynaecology
-
- MUELLER Andreas
- University Hospital Erlangen, Department of Obstetrics and Gynaecology
-
- BECKMANN Matthias W.
- University Hospital Erlangen, Department of Obstetrics and Gynaecology
-
- DITTRICH Ralf
- University Hospital Erlangen, Department of Obstetrics and Gynaecology
この論文をさがす
抄録
This study examines the possible predictive value of the LIVE/DEAD fluorescence viability assay for evaluation of survival of cryopreserved human ovarian tissue. Ovarian tissue from ten patients was examined by LIVE/DEAD viability staining before and after cryopreservation and after freezing in a -20 C freezer (negative control). After cryopreservation with a slow freezing protocol and cryoprotectant the LIVE/DEAD assay showed 86% viable follicles (an intact oocyte and at least more than 50% of the granulosa cells alive), whereas after freezing at -20 C the survival rate was 67%. The healthy follicular loss after cryopreservation was 4%, whereas with freezing at -20 C, it was 25%. Although this assay overestimates the survival rate of cryopreserved primordial follicles, if the LIVE/DEAD assay yields greater than approximately 85% viable follicles, it can be assumed that the follicles in the cryopreserved tissue have maintained their developmental potential and that the tissue is suitable for retransplantation.<br>
収録刊行物
-
- Journal of Reproduction and Development
-
Journal of Reproduction and Development 52 (4), 577-582, 2006
公益社団法人 日本繁殖生物学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001206336423552
-
- NII論文ID
- 10020351930
-
- NII書誌ID
- AA10936678
-
- ISSN
- 13484400
- 09168818
-
- NDL書誌ID
- 8020015
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可