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- AIKAWA Satoko
- Laboratory of Molecular Biology and Gene Regulation, Department of Life Science, School of Agriculture, Meiji University
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- SATO Takanobu
- Laboratory of Molecular Biology and Gene Regulation, Department of Life Science, School of Agriculture, Meiji University
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- ONO Tetsuo
- Laboratory of Molecular Biology and Gene Regulation, Department of Life Science, School of Agriculture, Meiji University
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- KATO Takako
- Laboratory of Molecular Biology and Gene Regulation, Department of Life Science, School of Agriculture, Meiji University
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- KATO Yukio
- Laboratory of Molecular Biology and Gene Regulation, Department of Life Science, School of Agriculture, Meiji University
書誌事項
- タイトル別名
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- High Level Expression of Prop-1 Gene in Gonadotropic Cell Lines
この論文をさがす
抄録
Prop-1 acts as an upstream regulator for the Pit-1 gene to induce development of Pit-1 lineage pituitary cell lines, GH-, PRL-, and TSH-producing cells, in the early stage of pituitary organogenesis. Furthermore, Prop-1 is presumed to be involved in the function of FSH/LH-producing cells, gonadotropes, since the defective Prop-1 gene shows hypogonadism. Recently, we reported evidence that Prop-1 directly regulates expression of the porcine FSHβ gene, thus providing a novel advance in understanding the function of Prop-1 in FSH/LH production and hypogonadism. This study was intended to demonstrate the expressions of Prop-1 gene in pituitary tumor-derived cell lines. RT-PCR analyses were conducted of Pit-1, glycoprotein α subunit (αGSU), GnRH receptor, and cyclophilin A (a ubiquitously expressing gene). We observed expression of the Pit-1 gene in αT1-1, TαT1, MtT/S, GH3, and TtT/GF cells, expression of the αGSU gene in αT1-1, αT3-1, LβT2, LβT4, TαT1, and GH3 cells, and expression of GnRH receptor gene in αT3-1, LβT2, LβT4, and GH3 cells, respectively. These expression profiles were in accord with their cell lineages, with only a few exceptions. To accurately measure the expression level of the Prop-1 gene, a quantitative analysis was performed using the real-time PCR method. This analysis demonstrated that the LβT2 and LβT4 gonadotrope cell lines, which express the FSHβ gene, express the Prop-1 gene. Taken together with our previous observation that Prop-1 is present in the adult porcine pituitary gonadotropes, Prop-1 might also be involved in development of gonadotropes and hormone production.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 52 (2), 195-201, 2006
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681313234688
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- NII論文ID
- 10020352555
- 130000054633
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- NII書誌ID
- AA10936678
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- COI
- 1:CAS:528:DC%2BD28XmtlKisLg%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 7886266
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- PubMed
- 16394624
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可