クローンウシ胎仔におけるLeptin遺伝子,POU5F1遺伝子CpGアイランドのエピジェネティック状況の解析

DOI 日本農学文献記事索引 Web Site Web Site 参考文献47件
  • KREMENSKOY Maksym
    Laboratory of Cellular Biochemistry, Animal Resource Science/Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • KREMENSKA Yuliya
    Laboratory of Cellular Biochemistry, Animal Resource Science/Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • SUZUKI Masako
    Laboratory of Cellular Biochemistry, Animal Resource Science/Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • IMAI Kei
    Department of Technology, National Livestock Breeding Center
  • TAKAHASHI Seiya
    Reproductive Cell Biology Laboratory, Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Science
  • HASHIZUME Kazuyoshi
    Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University
  • YAGI Shintaro
    Laboratory of Cellular Biochemistry, Animal Resource Science/Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • SHIOTA Kunio
    Laboratory of Cellular Biochemistry, Animal Resource Science/Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo

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タイトル別名
  • Epigenetic Characterization of the CpG Islands of Bovine Leptin and POU5F1 Genes in Cloned Bovine Fetuses

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Abnormal development and fetal loss during postimplantation period are concerns for production of nuclear transferred animals. Aberrant DNA methylation is one of the reasons for poor survival of cloned animals. In mammalian genome DNA, CpG islands are preferentially located at the start of transcription of housekeeping genes and are associated with tissue-specific genes. The correct and consecutive mechanisms of DNA methylation in the CpG islands are necessary for selective gene expressions that determine the properties of individual cells, tissues, and organs. In this study, we investigated the methylation status of the CpG islands of the bovine Leptin and POU5F1 genes in fetal and placental tissues from fetuses produced by artificial insemination (AI) and nuclear transfer (NT) at days 48 and 59 of pregnancy. Altered DNA methylation was observed in the normal and cloned fetal, placental, and endometrial tissues using bisulfite sequencing and pyrosequencing. Different tissue-specific methylated regions in the bovine Leptin and POU5F1 genes show a variable methylation status in NT fetuses compared to AI control.<br>

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