Induction of Hemagglutinating Activity of Edwardsiella tarda by Sodium Chloride

• Yasunobu Hideki

  Fisheries Technology Institute, Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries

• Arikawa Yoko

  Graduate School of Biosphere Science, Hiroshima University

• Furutsuka-Uozumi Kaori

  Fisheries Technology Institute, Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries

• Dombo Munehiko

  Research and Development Center, Unitika Ltd.

• Iida Takaji

  National Research Institute of Aquaculture, Fisheries Research Agency

• Mahmoud Mahmoud Mostafa

  Graduate School of Biosphere Science, Hiroshima University

• Okuda Jun

  Graduate School of Biosphere Science, Hiroshima University

• Nakai Toshihiro

  Graduate School of Biosphere Science, Hiroshima University

Bibliographic Information

Other Title

• <i>Edwardsiella tarda</i> の赤血球凝集活性に及ぼす培地塩分濃度の影響

Abstract

The hemagglutinating activity (HA) of Edwardsiella tarda, which had been isolated from cultured fish and culture environments, was investigated in relation to NaCl concentration of the growth medium. E. tarda cells were cultured in a peptone-yeast extract broth supplemented with 3% NaCl (3%-NaCl culture) and without NaCl (0%-NaCl culture). Hemagglutination assays with guinea pig erythrocytes classified the strains into three HA patterns. Seventeen strains exhibited HA only with the 3%-NaCl culture (type A). A more frequent type (35 strains) displayed HA in both 0%- and 3%- NaCl cultures but the 3%-NaCl culture showed higher HA activity than the 0%-NaCl culture (type B). No HA was detected in both cultures of the other three strains (type C). The NaCl-induced HA was well correlated with the expression of a 19.3 kDa protein, a fimbrial major subunit (FimA). Infection experiments with a selected strain (type A) of E. tarda revealed that the 3%-NaCl culture was more virulent to Japanese flounder Paralichthys olivaceus than the 0%-NaCl culture, when fish were challenged by an oral route. This induction of the fimbrial protein under higher NaCl concentration may play an important role in the virulence of E. tarda in marine environments.

Journal

• Fish Pathology

  Fish Pathology 41 (1), 29-34, 2006

  The Japanese Society of Fish Pathology

Keywords

• hemagglutination
• <i>Edwardsiella tarda</i>
• pathogenicity
• fimbriae
• <i>Paralichthys olivaceus</i>

Details

• CRID: 1390001206462134272
• NII Article ID: 120001065346
• NII Article ID: 10020355727
• NII Book ID: AN00063165
• DOI: 10.3147/jsfp.41.29
• COI: 1:CAS:528:DC%2BD28XkvVKrtbg%3D
• ISSN: 18817335
• ISSN: 0388788X
• NDL BIB ID: 8056244
• Web Site: http://ir.lib.hiroshima-u.ac.jp/00025669
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8056244
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed