Refractory Factors in Head and Neck Cancer: ATP Binding Cassette Transporters Expressed in Head and Neck Cancer Cell Lines

• Uematsu Takashi

  Department of Oral and Maxillofacial Surgery, Matsumoto Dental University School of Dentistry Institute for Oral Science, Matsumoto Dental University School of Dentistry

• Naramoto Hiroko

  Department of Oral and Maxillofacial Surgery, Matsumoto Dental University School of Dentistry

• Doto Ryosuke

  Department of Oral and Maxillofacial Surgery, Matsumoto Dental University School of Dentistry

• Uchihashi Takayuki

  Department of Oral and Maxillofacial Surgery, Matsumoto Dental University School of Dentistry

• Matsuura Takashi

  Department of Oral and Maxillofacial Surgery, Matsumoto Dental University School of Dentistry

• Usui Yohei

  Department of Orthodontics, Matsumoto Dental University School of Dentistry

• Uematsu Setsuko

  Department of Orthodontics, Matsumoto Dental University School of Dentistry

• Li Xianqi

  Institute for Oral Science, Matsumoto Dental University School of Dentistry

• Takahashi Masahiro

  Department of Oral and Maxillofacial Surgery, Matsumoto Dental University School of Dentistry

• Yamaoka Minoru

  Department of Oral and Maxillofacial Surgery, Matsumoto Dental University School of Dentistry

• Furusawa Kiyofumi

  Department of Oral and Maxillofacial Surgery, Matsumoto Dental University School of Dentistry Institute for Oral Science, Matsumoto Dental University School of Dentistry

Abstract

The aim of the present study was to clarify whether ATP binding cassette transporters are refractory factors in head and neck cancer chemotherapy. For in vitro and in vivo chemotherapeutic studies, we employed a human salivary gland adenocarcinoma cell line (HSY) and a human oral squamous cell carcinoma cell line (SCCSK) with vincristine (VCR) at clinically equivalent doses. Western blot analysis, reverse transcription-polymerase chain reaction, in vivo evaluation in xenograft models inoculated with cultured carcinoma cell line and drug efflux analysis were performed. VCR-treated SCCSK and HSY cells, as well as xenografted SCCSK and HSY cells in tumor-bearing nude mice, were found to express MDR1/ABCB1 and MRP1/ ABCC1. In addition to MDR1 and MRP1 mRNA, HSY/VCR and its cloned cells expressed MRP7/ABCC10 mRNA, but SCCSK/VCR did not express MRP7. Furthermore, drug resistance to VCR and docetaxel decreased in HSY/VCR in the presence of a competitive MRP7 inhibitor, 17-beta-estradiol-(17-beta-D-glucuronide). These results indicate that MDR1 and MRP1 expression are refractory factors in head and neck cancer chemotherapy and suggest that induction of MRP7 expression is involved in drug resistance in salivary gland adenocarcinomas.

Journal

• Oral Science International

  Oral Science International 3 (2), 72-83, 2006

  Japanese Stomatological Society

Keywords

• multidrug resistance
• squamous cell carcinoma
• salivary gland cell adenocarcinoma
• ABC transporter
• MDR1
• MRP1
• MRP7

Details

• CRID: 1390282680198928000
• NII Article ID: 130004503828
• NII Article ID: 10020357283
• NII Book ID: AA1196972X
• DOI: 10.11277/osi.3.72
• ISSN: 18814204
• ISSN: 13488643
• Web Site: https://search.jamas.or.jp/link/ui/2007217119
• Text Lang: en
• Data Source:
  • JaLC
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed