Ｈｕｍａｎ　Ｉｍｍｕｎｏｄｅｆｉｃｉｅｎｃｙ　Ｖｉｒｕｓ　Ｔｙｐｅ　１　ＲＮＡ定量における現行法（アンプリコアＨＶＩ‐１モニターＶｅｒ１．０）とアド・イン・プライマー法の解離に関する検討

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  • Comparison in Quantification of Human Immunodeficiency Virus Type 1 RNA Measured by Standard Method (Amplicor HIV Monitor Test Ver.1.0) and Add-in Primer Mix Method
  • Human Immunodeficiency Virus Type 1 RNA テイリョウ ニ オケル ゲンコウホウ アンプリコア HIV 1 モニター Ver 1 0 ト アド イン プライマーホウ ノ カイリ ニ カンスル ケントウ

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Objective: The level of human immunodeficiency virus type 1 (HIV-1) RNA in plasma is one of the important parameters in clinical practices for HIV-1 infected patients. How ver, it has been suggested that the standard method (Amplicor HIV Monitor Test Ver1.0) is less sensitive to non-subtype B than subtype B HIV-1. We therefore examined the usefulness of the add-in primer mix method using samples obtained from Japanese HIV-1 infected patients.<BR>Method: We compared the sensitivity of the standard method and that of a modified method with an add-in primer, using plasma samples obtained from 12 seropositive hemophiliacs and 82 seropositive non-hemophiliacs.<BR>Results: Cross-reactivity was less than 12.1% on average in 17 (32.1%) of the 53 non-hemophiliac samples when comparing standard and modified methods, while the HIV-1 RNA levels determined with these two methods were comparable in the 12 hemophiliacs.<BR>Conclusion: These results indicate that the HIV-1 RNA assay system, which has high sensitivity not only to subtype B but also to non-subtype B HIV-1, is inevitable in Japan.

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