The W chromosome detection in several lepidopteran species by genomic in situ hybridization (GISH)

  • Yoshido Atsuo
    Laboratory of Applied Molecular Entomology, Graduate School of Agriculture, Hokkaido University
  • Yamada Yasuhiro
    Field Science Center for Northern Biosphere, Hokkaido University
  • Sahara Ken
    Laboratory of Applied Molecular Entomology, Graduate School of Agriculture, Hokkaido University

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タイトル別名
  • The W Chromosome Detection in Several Lepidopteran Species by Genomic <i>in situ</i> Hybridization (GISH)
  • W chromosome detection in several lepidopteran species by genomic in situ hybridization GISH

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抄録

We newly identified the W chromosome in eight lepidopteran species and confirmed the absence of W chromosome in Caligula japonica females with the Z0 sex chromosome constitution by means of genomic in situ hybridization (GISH). In pachytene oocytes of species with the WZ/ZZ sex chromosome system, female-derived genomic probes of the respective species highlighted the whole W chromosome thread in the WZ bivalent and also stained specific autosomal region(s) with telomeric and/or subtelomeric signals. On the basis of GISH results obtained in this study and earlier published, we classified karyotypes of lepidopteran species into three types.<br>

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