Rapid ELISA in Droplet on PDMS Dimple with Nanoliter Reagents Dispensed by Ink-jet Microchip

• Kudo Yuki

  Faculty of Urban Environmental Sciences, Tokyo Metropolitan University

• Nishiwaki Moeka

  Faculty of Urban Environmental Sciences, Tokyo Metropolitan University

• Inoue Yoriko

  Faculty of Urban Environmental Sciences, Tokyo Metropolitan University

• Seino Nobuko

  Faculty of Urban Environmental Sciences, Tokyo Metropolitan University

• Nakagama Tatsuro

  Faculty of Urban Environmental Sciences, Tokyo Metropolitan University

• Uchiyama Katsumi

  Faculty of Urban Environmental Sciences, Tokyo Metropolitan University

抄録

IgA ELISA was performed in a droplet using an ink-jet microchip for shortening the reaction time, saving the reaction reagents. Forty-five dimples (500-μm i.d., 100-μm depth) were prepared on a PDMS (polydimethylsiloxane) microchip plate (30×30 mm) for the reaction vessels. After the dimple was pored with nanoliter water containing glycerin, an antibody solution was firstly added for immobilization on the dimple, and then nanoliter reaction regents for ELISA (enzyme-linked immunosorbent assay) were added sequentially using an ink-jet chip. A good linear relationship between the fluorescence intensity of Ample^®Red and concentration of IgA was obtained with each reaction time of 150 s.

収録刊行物

• Chemistry Letters

  Chemistry Letters 37 (5), 536-537, 2008

  公益社団法人 日本化学会

キーワード

• General Chemistry

詳細情報

• CRID: 1390001204586228608
• NII論文ID: 10021080491
• NII書誌ID: AA00603318
• DOI: 10.1246/cl.2008.536
• ISSN: 13480715
• ISSN: 03667022
• Web Site: https://academic.oup.com/chemlett/article-pdf/37/5/536/55595649/cl.2008.536.pdf
• 本文言語コード: en
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