Quantitative Analysis of Binding Affinities of Biological Macromolecular Complexes by Laser Spray Mass Spectrometry

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  • AKASHI Satoko
    Yokohama City University, International Graduate School of Arts and Sciences, Supramolecular Biology
  • SHI Xiangguo
    Yokohama City University, International Graduate School of Arts and Sciences, Supramolecular Biology
  • TAKAMIZAWA Atsushi
    University of Yamanashi, Clean Energy Research Center
  • NISHIMURA Yoshifumi
    Yokohama City University, International Graduate School of Arts and Sciences, Supramolecular Biology
  • HIRAOKA Kenzo
    University of Yamanashi, Clean Energy Research Center

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  • レーザースプレー質量分析が可能にした生体高分子複合体の結合親和性の定量的な解析
  • レーザー スプレー シツリョウ ブンセキ ガ カノウ ニ シタ セイタイ コウブンシ フクゴウタイ ノ ケツゴウ シンワセイ ノ テイリョウテキナ カイセキ

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Abstract

This commentary describes the advantages of laser spray, which was developed by Hiraoka et al., in the analysis of binding affinities of biomolecular complexes, such as DNA-protein and DNA-drug complexes. Since laser spray enables dissociation of the complexes without fragmentation of labile compounds such as DNA, a good correlation was found between the binding affinities and applied laser power for dissociation. We found that laser spray is promising for rapid characterization of binding affinities of complexes with small differences in structure.

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