DNA Microarray Analysis of Temporal and Spatial Variation of Bacterial Communities in Japanese Rivers

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  • UPADHYE RAHUL R.
    Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University
  • INOUE DAISUKE
    Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University
  • INABA MASAKI
    Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University
  • SEI KAZUNARI
    Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University
  • IKE MICHIHIKO
    Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University

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  • DNAマイクロアレイを用いた日本の河川水中における細菌群集の時空間的変動の解析

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Abstract

To determine the spatial and seasonal variations in bacterial community structure and abundance in the small and steep rivers typically present in Japan, bacterial populations in two rivers, the Yodo River and Kita River, were investigated using a DNA microarray technique. A total of 24 river water samples seasonally collected from four stations in the Yodo River and two stations in the Kita River were analyzed by an oligonucleotide DNA microarray targeting the conserved region of 16S rDNA in 1016 bacterial species. The phyla Proteobacteria, Firmicutes, Actinobacteria, Cyanobacteria, and Bacteroidetes were the dominant bacterial groups in the river water samples investigated, and alpha-Proteobacteria appeared to be the most dominant among the Proteobacteria. Overall diversity, composition and shifts in the bacterial communities depended mainly on the season. Pollution level (as indicated by nutrient concentration) and specific bacterial sources, such as effluent from wastewater treatment plants and backflow of seawater, also appeared to influence bacterial community structure in these small, fast-flowing rivers.

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