Live Single-Cell Molecular Analysis by Video-Mass Spectrometry

• TSUYAMA Naohiro

  Graduate School of Biomedical Sciences, Hiroshima University

• MIZUNO Hajime

  Graduate School of Biomedical Sciences, Hiroshima University

• TOKUNAGA Emi

  Graduate School of Biomedical Sciences, Hiroshima University

• MASUJIMA Tsutomu

  Graduate School of Biomedical Sciences, Hiroshima University

抄録

The direct molecular analysis of a live single cell viewed under a video-microscope has been developed. The cell contents are sucked into a nano-electrospray tip, and hundreds of peaks of ionic compounds of low molecular weight are detected by nano-ESI Q-TOF mass spectrometry (MS). Cell-specific MS peaks in a single mouse-embryonic fibroblasts cell are extracted by a t-test, and one of the peaks is proceeded to MS/MS analysis for molecular identification. This method is direct and quick to identify the molecules of a cell with simultaneous observation by a video-microscope.

収録刊行物

• Analytical Sciences

  Analytical Sciences 24 (5), 559-561, 2008

  社団法人 日本分析化学会

キーワード

• Analytical Chemistry

詳細情報

• CRID: 1390282679233421568
• NII論文ID: 130004441643
• NII論文ID: 10021099872
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.24.559
• COI: 1:CAS:528:DC%2BD1cXnvFCrs7k%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 9482982
• PubMed: 18469458
• Web Site: http://id.ndl.go.jp/bib/9482982
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9482982
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.24.559.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.24.559/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/24/5/24_5_559/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
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