Optimization of Conditions for High-Performance Size-Exclusion Chromatography of Different Soil Humic Acids

• ASAKAWA Daichi

  The Graduate School of Agricultural Science, Kobe University

• KIYOTA Takuro

  The Graduate School of Science and Technology, Kobe University

• YANAGI Yukiko

  The Graduate School of Agricultural Science, Kobe University

• FUJITAKE Nobuhide

  The Graduate School of Agricultural Science, Kobe University

Bibliographic Information

Other Title

• Development and validation of method of high-performance size-exclusion chromatography (HPSEC) for various soil humic acids

Abstract

A method of high-performance size-exclusion chromatography (HPSEC) for a wide variety of soil humic acids (HAs) was developed. Two types of soil HAs (Cambisol and Andosol HAs), which have substantially different chemical properties, showed different effects of salt and organic solvent concentrations in the eluent on chromatograms. A Shodex OHpak SB-805 HQ column with 10 mM sodium phosphate buffer (pH 7.0) containing 25% of acetonitrile (v/v) was found to be applicable for different HAs, and showed high reproducibility and recovery (87.0 - 94.5%). The Cambisol HA was fractionated into five fractions using an ultrafiltration with different molecular-weight cut-offs. The order of the molecular weights of the five fractions calculated from the HPSEC analysis corresponded to that defined by ultrafiltration. This supported the reliability of the method.

Journal

• Analytical Sciences

  Analytical Sciences 24 (5), 607-613, 2008

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390001204256669824
• NII Article ID: 130004441651
• NII Article ID: 10021100065
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.24.607
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 9483045
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9483045
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.24.607.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.24.607/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/24/5/24_5_607/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed