Size-Effects in the Separation of Bovine Serum Albumin through an Aqueous Polymer Two-Phase Systems on the Micron-Size Devices
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- Kuwabara Kazuhiro
- Department of Chemical Engineering, Tokyo Institute of Technology
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- Ohtaguchi Kazuhisa
- Department of Chemical Engineering, Tokyo Institute of Technology
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The effect of device-downsizing upon the continuous extraction of bovine serum albumin (BSA) in an aqueous two-phase system was studied. BSA molecules in the aqueous solution of polyethylene glycol (PEG) were partitioned into that of dextran (Dex) through μm- or mm-sized two-phase flow systems in circular capillary tubes. Mathematical model of BSA extraction kinetics was formulated with dimensionless forms. Four effects of device-downsizing were observed. First, downsizing enlarged the specific interfacial area between a PEG-rich solution and a Dex-rich solution, and in turn, elevated overall volumetric mass transfer rates of BSA in the PEG-rich phase and in the Dex-rich phase. Second, downsizing enlarged the proportion of the cross section of the PEG-rich solution, and in turn, elevated the space-time of the PEG-rich phase flow. Third, downsizing reduced the delay space-time of mass transfer of BSA into the Dex-rich solution. Finally, downsizing enlarged the range of overall volumetric mass transfer rates of BSA extraction. The diminution in size of two-phase flow separator towards micron-size was found to be effective to increase the level of overall volumetric mass transfer rates of BSA extraction.
収録刊行物
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- JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
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JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 41 (2), 114-120, 2008
公益社団法人 化学工学会
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詳細情報 詳細情報について
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- CRID
- 1390001204569836416
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- NII論文ID
- 10021109711
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- NII書誌ID
- AA00709658
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- ISSN
- 18811299
- 00219592
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- NDL書誌ID
- 9384037
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可