Micro-Flow System Comprised of a Fused-Silica Capillary and Chemiluminescence Detection that Works under Laminar Flow Conditions

DOI Web Site Web Site 被引用文献5件 参考文献24件
  • Tsukagoshi Kazuhiko
    Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
  • Ishida Shingo
    Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
  • Nakajima Riichiro
    Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University

この論文をさがす

抄録

A micro-flow system comprised of a fused-silica capillary and chemiluminescence detection was developed, in which a wide-bore hydrodynamic chromatography mode was first introduced into the system. The micro-flow system was run with a gravity method or pump flow method for feeding a carrier solution into the capillary. Oxalate–hydrogen peroxide chemiluminescence reaction was adopted as a detection technique. Fluorescent compound (fluorescein isothiocyanate; FITC) and FluoSphere fluorescent polymer particles with particle diameters of 0.02 μm and 1.0 μm were used as model samples in the present system. FITC and FluoSpheres (0.02 μm) with diffusion indexes greater than ca. 1 were delivered into the capillary with average linear velocities under laminar flow conditions, which led to a Gaussian peak on the chemiluminescence profile. On the other hand, FluoSpheres (1.0 μm) with a diffusion index of ca. 0.1 were delivered with the approximately maximum linear velocity and formed a non-Gaussian peak. The mixtures, FITC and FluoSpheres (1.0 μm) as well as FluoSpheres (0.02 μm) and FluoSpheres (1.0 μm), reflected the individual diffusion indexes, indicating two main peaks on the chemiluminescence profiles. An unexpected small Gaussian peak observed with the FluoSpheres (1.0 μm) sample was also observed.

収録刊行物

被引用文献 (5)*注記

もっと見る

参考文献 (24)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ