Developmental Changes in Spontaneous Beating Rhythm of Cardiac Myocytes in vitro Cultured with Molecular Diffusion Culture Method
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- Takeuchi Akimasa
- School of Allied Health Sciences, Kitasato University
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- Ogawa Hajime
- Graduate School of Frontire Sciences, University of Tokyo
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- Moriguchi Hiroyuki
- Graduate School of Frontire Sciences, University of Tokyo
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- Lee Jong-Kook
- Research Institute of Environmental Medicine, Nagoya University
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- Noshiro Makoto
- School of Allied Health Sciences, Kitasato University
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- Kotani Kiyoshi
- Graduate School of Frontire Sciences, University of Tokyo
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- Jimbo Yasuhiko
- Graduate School of Frontire Sciences, University of Tokyo
Bibliographic Information
- Other Title
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- 分子拡散培養法を用いた培養心筋細胞の成長過程における自律拍動リズムの時間変化
- ブンシ カクサン バイヨウホウ オ モチイタ バイヨウ シンキン サイボウ ノ セイチョウ カテイ ニ オケル ジリツ ハクドウ リズム ノ ジカン ヘンカ
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Abstract
To understand the detailed mechanisms of arrhythmia and its treatment methods, it is required to elucidate the mechanisms of formation and debacle of cardiac beat at a microscopic level by using appropriate in vitro models of cardiac beating. In this study, we have developed a method for an medium-exchange-free culture of rat cardiac myocytes, and carried out long-term recording of electrical activity of cardiac myocytes from postnatal rat by means of microelectrode-array (MEA) and the new culture method. Figure 6 a) ∼ f) is a series of the properties of spontaneous beating (mean beating interval, standard deviation and coefficient of variation) of cardiac myocytes cultured by conventional culture method with alternate-day medium exchange (a ∼ c) or newly developed “molecular diffusion culture” method (d ∼ f). As shown in these figures, the difference of the beating property between the individual cultures, which is observed in conventional cultures (a) ∼ c)), was dramatically reduced in the molecular diffusion cultures (d) ∼ f)). In addition, these results also indicated that there is a transient period when all of mean beating interval, standard deviation, and coefficient of variation increase transiently before the formation of stable rhythmic fast beat.
Journal
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- IEEJ Transactions on Electronics, Information and Systems
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IEEJ Transactions on Electronics, Information and Systems 128 (7), 1064-1069, 2008
The Institute of Electrical Engineers of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390001204605054208
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- NII Article ID
- 10021133030
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- NII Book ID
- AN10065950
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- ISSN
- 13488155
- 03854221
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- NDL BIB ID
- 9563981
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed