NO_3^-およびNO_2^-を電子受容体としたメタノール脱窒リアクターの運転とその脱窒細菌の遺伝子の解析  [in Japanese] Operation of a Methanol Denitrification Reactor Fed with NO_3^- and NO_2^- as Electron Acceptors and Analysis of Denitrifying Bacterial Genes  [in Japanese]

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Author(s)

    • 新田見 匡 NITTAMI Tadashi
    • 横浜国立大学大学院工学研究院機能の創生部門 Division of Materials Science and Chemical Engineering, Graduate School of Engineering, Yokohama National University
    • 今井 裕子 IMAI Yuko
    • 横浜国立大学大学院工学府機能発現工学専攻 Division of Materials Science and Chemical Engineering, Graduate School of Engineering, Yokohama National University
    • 中村 一穂 [他] NAKAMURA Kazuho
    • 横浜国立大学大学院工学研究院機能の創生部門 Division of Materials Science and Chemical Engineering, Graduate School of Engineering, Yokohama National University
    • 松本 幹治 MATSUMOTO Kanji
    • 横浜国立大学大学院工学研究院機能の創生部門 Division of Materials Science and Chemical Engineering, Graduate School of Engineering, Yokohama National University

Abstract

We compared the denitrification rates and bacterial denitrification genes (<i>nirK</i> and <i>nirS</i>) between denitrification processes using NO<sub>2</sub><sup>-</sup> and NO<sub>3</sub><sup>-</sup>. We operated a sequencing batch reactor (SBR) for denitrification using activated sludge. The SBR was first fed with NO<sub>3</sub><sup>-</sup> (Run 1), and the electron acceptor was then changed to NO<sub>2</sub><sup>-</sup> (Run 2). Methanol was fed as the major electron donor through out the operational period (64 days). Denitrification rates (mg-N · g-MLSS<sup>-1</sup> · h<sup>-1</sup>) were measured at regular intervals. Results showed that the maximum denitrification rates were more than 40 mg-N · g-MLSS<sup>-1</sup> · h<sup>-1</sup> irrespective of electron acceptor type. However, it takes 12 days to reach the maximum denitrification rate after changing the electron acceptor to NO<sub>2</sub><sup>-</sup>. The results of the cloning analysis of <i>nirK</i> and <i>nirS</i> implied that the lag time was attributable to bacterial population shifts, because the <i>nirK</i> and <i>nirS</i> detected at Run 1 were phylogenetically different from those at Run 2. However, the changes in the total copy numbers of <i>nirK</i> or <i>nirS</i> could not explain the changes in the denitrifying rates.

Journal

  • Journal of Japan Society on Water Environment

    Journal of Japan Society on Water Environment 31(5), 269-274, 2008-05-10

    Japan Society on Water Environment

References:  26

Codes

  • NII Article ID (NAID)
    10021158660
  • NII NACSIS-CAT ID (NCID)
    AN10372439
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    09168958
  • NDL Article ID
    9506726
  • NDL Source Classification
    ZN5(科学技術--建設工学・建設業--都市工学・衛生工学)
  • NDL Call No.
    Z16-1045
  • Data Source
    CJP  NDL  J-STAGE 
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