Production of Succinic Acid by Lactobacillus plantarum Using Metabolic Rerouting

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  • SATOH Eiichi
    Department of Applied Biology and Chemistry, Tokyo University of Agriculture
  • ENDO Michio
    Department of Applied Biology and Chemistry, Tokyo University of Agriculture Graduation School of Agricultural and Life Sciences, The University of Tokyo
  • OKADA Sanae
    Department of Applied Biology and Chemistry, Tokyo University of Agriculture
  • UCHIMURA Tai
    Department of Applied Biology and Chemistry, Tokyo University of Agriculture
  • HOLS Pascal
    Université Catholique de Louvain, Institut des Sciences de la Vie, Unit de Gé né tique Place Croix du Sud

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Other Title
  • 代謝改変を利用したＬａｃｔｏｂａｃｉｌｌｕｓ　ｐｌａｎｔａｒｕｍによるコハク酸生産
  • タイシャ カイヘン オ リヨウシタ Lactobacillus plantarum ニ ヨル コハクサン セイサン

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Abstract

Lactobacillus plantarum NCIMB 8826 strain was used as a model for succinic acid production by means of metabolic rerouting. Since this strain is the same as L. plantarum WCFS 1 whose genome has been completely analyzed, it is suggested that NCIMB 8826 strain possesses a potential succinic acid biosynthesis pathway. NCIMB 8826 strain produced a small mount of succinic acid grown in MRS medium in the presence of sodium bicarbonate. This means that the succinic acid biosynthesis pathway of this strain functions. In order to improve the potential of the succinic acid production, a pyruvate carboxylase of this strain was over in NCIMB 8826. However no remarkable increase of succinic acid production was observed, and the main product was still lactic acid. When a D-LHD and L-LDH double knocked-out mutant, which is a derivative mutant from NCIMB 8826 strain, was grown in MRS medium in the presence of sodium bicarbonate, a 12-fold amount of succinic acid was produced compared with that produced by the wild strain.

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