Application of Real Time PCR for the Quantitative Detection of Radiation-induced Genomic DNA Strand Breaks

• Yamauchi Emiko

  Division of Radiological Protection and Biology, National Institute of Infectious Diseases Department of Biological Production, Tokyo University of Agriculture and Technology

• Watanabe Ritsuko

  Radiation Effect Analysis Group, Japan Atomic Energy Agency

• Oikawa Miyoko

  Division of Radiological Protection and Biology, National Institute of Infectious Diseases Division of Science and Technology, Shinshu University

• Fujimoto Hirofumi

  Division of Radiological Protection and Biology, National Institute of Infectious Diseases

• Yamada Akinori

  Center of Molecular Biosciences, University of Ryukyus

• Saito Kimiaki

  Radiation Effect Analysis Group, Japan Atomic Energy Agency

• Murakami Masahiro

  Radiation Hazard Research Group, National Institute of Radiological Sciences

• Hashido Kazuo

  Division of Radiological Protection and Biology, National Institute of Infectious Diseases

• Tsuchida Kozo

  Division of Radiological Protection and Biology, National Institute of Infectious Diseases

• Takada Naoko

  Division of Radiological Protection and Biology, National Institute of Infectious Diseases

• Fugo Hajime

  Department of Biological Production, Tokyo University of Agriculture and Technology

• Tu Zhenli

  College of Animal Sciences, Zhejiang University

• Maekawa Hideaki

  Division of Radiological Protection and Biology, National Institute of Infectious Diseases

Abstract

The frequency of single strand breaks (SSBs) occurring on both strands of the pBR322 plasmid DNA region flanked by a pair of primers used for polymerase chain reaction (PCR) amplifications was determined after irradiation with ¹³⁷Cs γ rays. We verified that real time PCR is suitable for the detection and quantitative analysis of SSBs caused by γ ray irradiation. The utility of this approach was also supported by the comparison of the practical experimental data with the Monte Carlo simulation. The potential application of this PCR method for the detection of genomic DNA damage was also confirmed.<br>

Journal

• Journal of Insect Biotechnology and Sericology

  Journal of Insect Biotechnology and Sericology 77 (1), 1_17-1_24, 2008

  The Japanese Society of Sericultural Science

Keywords

• single strand break
• radiation
• scavenger
• real-time PCR
• plasmid DNA
• Monte Carlo simulation
• <i>Bombyx mori</i>

Details

• CRID: 1390282680173907712
• NII Article ID: 10021220872
• NII Book ID: AA11558849
• DOI: 10.11416/jibs.77.1_17
• ISSN: 18847978
• ISSN: 13468073
• NDL BIB ID: 9400339
• Web Site: http://id.ndl.go.jp/bib/9400339
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9400339
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • CiNii Articles
• Abstract License Flag: Disallowed