Susceptibility of Newly Established Cell Lines from Helicoverpa armigera to Homologous and Heterologous Nucleopolyhedroviruses





A total of five new cell lines were established from Helicoverpa armigera; including KU-HaEmb1 (HaEmb1) and KU-HaEmb2 (HaEmb2) from embryos, KU-HaPO1 (HaPO1) and KU-HaPO2 (HaPO2) from pupal ovaries, and KU-HaAO1 (HaAO1) from adult ovaries. These cell lines were examined for their permissiveness for homologous H. armigera nucleopolyhedrovirus (HearNPV) and seven heterologous NPVs. Upon infection with HearNPV, the cell line HaAO1 yielded the highest productivity in terms of amounts of polyhedra, budded virus (BV), polyhedrin and viral DNA among the six cell lines, including the five newly established cell lines and the cell line from Helicoverpa zea (BCIRL-Hz-AM1), which is commonly used for in vitro studies and production of HearNPV. The cell lines HaPO1, HaPO2 and HaEmb2 were also permissive for HearNPV but they yielded lower productivity than that of the cell line HaAO1. Only the cell line HaEmb1 was nonpermissive for HearNPV infection among the cell lines established. Examination with seven heterologous NPVs showed that all the new cell lines continued to proliferate without any cytopathic effects (CPE), following infection with five NPVs from <i>Bombyx mori</i>, Hyphantria cunea, Lymantria dispar, Orgyia pseudotsugata and Spodoptera litura. Upon infection with NPVs from Autographa californica (AcMNPV) and Spodoptera exigua (SeMNPV), all the new cell lines, with the exception of HaAO1, manifested CPE to varying degrees, synthesizing significant amount of viral DNA. These cell lines, with the exception of AcMNPV-infected HaPO1 that produced significant amount of BVs, yielded negligible or very low level of BVs and polyhedrin following infection with AcMNPV and SeMNPV, indicating that they were semipermissive for these two NPVs. These results indicate that the newly established H. armigera cell line HaAO1 provide a promising candidate for in vitro production of HearNPV biopesticide, as well as a useful system for the analysis of molecular mechanisms of NPV-cell interactions.<br>


  • Journal of insect biotechnology and sericology

    Journal of insect biotechnology and sericology 77(1), 25-34, 2008-02-28

    社団法人 日本蚕糸学会

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