Development a long-term storage method for diapause eggs in some hybrid races of Bombyx mori

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  • Iizuka Tetsuya
    Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences
  • Mase Keisuke
    Silk Technology Unit, National Institute of Agrobiological Sciences
  • Okada Eiji
    Silk Technology Unit, National Institute of Agrobiological Sciences
  • Yamamoto Toshio
    Silk Technology Unit, National Institute of Agrobiological Sciences

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  • Development a long-term storage method for diapause eggs in some hybrid races of <i>Bombyx mori</i>

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Maintaining silkworm races can probe to be labor intensive because stock races must be reared annually. The most effective way of reducing labor is to extend the period of preservation. A method for rearing silkworms bi-annually was recently proposed; however, it required several complicated procedures for egg preservation. In this study, we developed a simpler method for the preservation of silkworm diapause eggs, involving minimum care, refrigeration, and without terminating diapause. We first optimized storage conditions by investigating the relationship between the long-term storage of diapause eggs and the number of days needed to achieve diapause at 25°C, as well as the number of days required for termination at 5°C. We then developed a very simple 2-year egg storage method, using a high-humidity refrigerator for egg storage and only one intermediate care procedure. Using this new method, the period from one generation to the next is now 2years.<br>

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