Virulence of Phospholipase C Mutants of <i>Pseudomonas aeruginosa</i> PAO1 against the Silkworm, <i>Bombyx mori</i>

• Iiyama Kazuhiro

  Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University

• Chieda Yuuka

  Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University

• Man Lee Jae

  Laboratory of Silkworm Science, Faculty of Agriculture, Graduate School, Kyushu University

• Kusakabe Takahiro

  Laboratory of Silkworm Science, Faculty of Agriculture, Graduate School, Kyushu University

• Yasunaga-Aoki Chisa

  Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University

• Shimizu Susumu

  Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University

Bibliographic Information

Other Title

• Virulence of phospholipase C mutants of Pseudomonas aeruginosa PAO1 against the silkworm, Bombyx mori

Abstract

To estimate the contribution of phospholipase C (PLC) in the virulence of Pseudomonas aeruginosa against Bombyx mori, PlcB, PlcH, and PlcN mutants were created by the sacB-based counterselection method. Insertion of Ωaac cassette (selection marker, Gm^R) in plcB, plcH, and plcN was confirmed by Southern hybridization and the mutants were designated as PAO1plcB, PAO1plcH, and PAO1plcN, respectively. Inactivation of PLC genes did not cause growth deficient in vitro. The culture filtrates of PAO1, PAO1plcB, and PAO1plcN had hemolytic activity, but not PAO1plcH. When the PLC mutants were inoculated into the fourth instar of Bombyx mori larvae, all mutants maintained high virulence, indicating that at least inactivation of a single plc gene in P. aeruginosa did not affect the virulence against B. mori. It was different from the results obtained in another Lepidopteran host model, Galleria mellonella.<br>

Journal

• Journal of Insect Biotechnology and Sericology

  Journal of Insect Biotechnology and Sericology 77 (2), 2_115-2_120, 2008

  The Japanese Society of Sericultural Science

Keywords

• Pseudomonas aeruginosa
• <i>Bombyx mori</i>
• phospholipase C
• plcB
• plcH
• plcN
• virulence

Details

• CRID: 1390282680173836544
• NII Article ID: 10021221206
• NII Book ID: AA11558849
• DOI: 10.11416/jibs.77.2_115
• ISSN: 18847978
• ISSN: 13468073
• NDL BIB ID: 9574043
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9574043
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • CiNii Articles
• Abstract License Flag: Disallowed