A simple, rapid and automated method to detect spontaneous and induced platelet clump with a hematology analyzer-basic and clinical studies-

• SHIMIZU Mie

  Department of Oriental Medicine and Center for Complementary and Alternative Medicine, Tokai University School of Medicine

• KOHARA Saori

  Department of Neurology, Tokai University School of Medicine

• YAMAMOTO Masahiro

  Yokohama Stroke and Brain Center

• SHINOHARA Yukito

  Center for Stroke and Neurological Diseases, Tokai University Tokyo Hospital

Bibliographic Information

Other Title

• Ｈｅｍａｔｏｌｏｇｙ　Ａｎａｌｙｚｅｒを用いた血小板自然および惹起凝集塊検出法―基礎的および臨床的検討―
• A simple, rapid and automated method to detect spontaneous and induced platelet clump with a hematology analyzer
• — basic and clinical studies —
• ―基礎的および臨床的検討―

Abstract

Background and Purpose: In order to evaluate platelet activation, we developed a simple and rapid method to detect clumps (aggregates) of platelets using a hematology analyzer. The purpose of this study is to undertake basic evaluation and to investigate the clinical usefulness of our newly modified method. Method: The measurements were done on EDTA-whole blood and citrated whole blood from patients with cerebral infarction, and from non-cerebrovascular disease (CVD) patients with or without risk factors for vascular diseases. The blood cell counts and scattergrams of platelets and white blood cells were displayed rapidly and automatically. Results: Platelet clumps were not observed in the citrated whole blood of healthy subjects. When the aggregation-inducing drugs were added to the citrated whole blood of healthy subjects, the platelets clumped dose-dependently. The appearance of platelet clumps was well correlated with ADP-induced aggregation and epinephrine-induced aggregation measured by a laser-scattering method. Aggregation was observed in 27 of 39 non-CVD patients with risk factors and 37 of 76 patients with cerebral infarction. Conclusion: The newly modified method using a hematology analyzer is useful for detecting platelet activation in vivo.

Journal

• Japanese Journal of Thrombosis and Hemostasis

  Japanese Journal of Thrombosis and Hemostasis 17 (2), 165-174, 2006

  The Japanese Society on Thrombosis and Hemostasis

Keywords

• Hematology analyzer
• platelet activation
• hyperaggregability
• ischemic cerebrovascular disease
• stroke pathophysiology

Details

• CRID: 1390282679422103680
• NII Article ID: 10021258639
• NII Book ID: AN10353762
• DOI: 10.2491/jjsth.17.165
• ISSN: 18808808
• ISSN: 09157441
• Web Site: https://search.jamas.or.jp/link/ui/2006190187
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed